Super-resolution imaging of mitochondrial cristae using a more hydrophobic far-red Si-rhodamine probe

Jing Sun; Xichuan Ge; Boya Jin; Shiyi Li; Yiwei Hou; Suyi Zhong; Zikang Yang; Peng Xi; Meiqi Li; Baoxiang Gao

doi:10.1039/d3cc04696f

Super-resolution imaging of mitochondrial cristae using a more hydrophobic far-red Si-rhodamine probe

Chem Commun (Camb). 2023 Oct 31;59(87):13038-13041. doi: 10.1039/d3cc04696f.

Authors

Jing Sun¹, Xichuan Ge¹, Boya Jin^{2

3}, Shiyi Li¹, Yiwei Hou^{2

3}, Suyi Zhong^{2

3}, Zikang Yang¹, Peng Xi^{2

3}, Meiqi Li⁴, Baoxiang Gao¹

Affiliations

¹ Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Materials Science, Hebei University, Baoding 071002, Hebei, China. bxgao@hbu.edu.cn.
² Department of Biomedical Engineering, College of Future Technology, Peking University, Beijing 100871, China. xipeng@pku.edu.cn.
³ National Biomedical Imaging Centre, Peking University, Beijing 100871, China.
⁴ School of Life Sciences, Peking University, Beijing, 100871, China. limeiqi@pku.edu.cn.

PMID: 37843422
DOI: 10.1039/d3cc04696f

Abstract

Mitochondrial probe SiRPFA was synthesized by attaching a long perfluoroalkyl chain on Si-rhodamine cationic dye. High lipophilicity endowed SiRPFA with mitochondrial membrane potential independent properties. Under stimulated emission depletion microscopy, SiRPFA clearly revealed changes in mitochondrial cristae morphology during autophagy induced by starvation or apoptosis.

MeSH terms

Membrane Potential, Mitochondrial
Microscopy, Fluorescence / methods
Mitochondria* / metabolism
Mitochondrial Membranes* / metabolism
Rhodamines / metabolism

Substances

Rhodamines