Prevalence and epidemiological characteristics of methicillin-resistant Staphylococcus aureus isolated from cattle in Bangalore India as a part of the One Health approach

Nimita Venugopal; Rituparna Tewari; Feroze A Ganaie; Susweta Mitra; Rajeswari Shome; Bibek R Shome

doi:10.1099/acmi.0.000627.v3

Prevalence and epidemiological characteristics of methicillin-resistant Staphylococcus aureus isolated from cattle in Bangalore India as a part of the One Health approach

Access Microbiol. 2023 Sep 13;5(9):000627.v3. doi: 10.1099/acmi.0.000627.v3. eCollection 2023.

Authors

Nimita Venugopal^{1

2}, Rituparna Tewari^{1

3}, Feroze A Ganaie^{1

4}, Susweta Mitra^{1

5}, Rajeswari Shome¹, Bibek R Shome¹

Affiliations

¹ ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bangalore, India.
² Present address: Department of Microbiology, M.S.Ramaiah College of Arts, Science, and Commerce, Bangalore, India.
³ Present address: Centre for Cellular and Molecular Platforms, UAS-GKVK Campus, Bangalore, India.
⁴ Present address: Department of Medicine, Division of Pulmonary, Allergy, and Critical care, University of Alabama at Birmingham, Birmingham, AL, USA.
⁵ Present address: School of Basic and Applied Sciences, Dayananda Sagar University, Bangalore, India.

Abstract

In India, limited studies are available on the epidemiological aspects of methicillin-resistant Staphylococcus aureus (MRSA) infections in both animal and human settings. Herein, we investigated the prevalence, antimicrobial resistance profile and molecular characteristics of MRSA isolates recovered from cattle using the One Health approach. Out of 66 mecA-positive staphylococci, species-specific multiplex PCR detected 24 % (n=16) of isolates as MRSA. Maximum antibiotic resistance was seen against cloxacillin (94 %, n=15) and least for enrofloxacin and cephalothin (each 13 %, n=2). Overall, 13 % (n=2) of MRSA isolates were multidrug-resistant. Molecular characterization by SCCmec typing identified 88 % (n=14) of MRSA isolates as type V. Twelve isolates (75 %) belonged to novel spa-type t17242, of which 67 % (n=8) belonged to agr type I. MLST analysis revealed ST 1687 (50 %, n=8) as the most predominant sequence type. Circulation of different MRSA clones among the cattle populace offers a risk of transmission to humans through direct contact, food chain or environmental contamination. Thus, continuous monitoring of MRSA strains is imperative for early diagnosis and for establishing effective treatment strategies to restrain the disease burden caused by MRSA infections.

Keywords: SCCmec typing; methicillin-resistant Staphylococcus aureus; multidrug resistance; multilocus sequence typing; one health; spa typing.