Self-powered biosensor using photoactive ternary nanocomposite: Testing the phospholipid content in rhodotorula glutinis oil

Weining Wang; Hairong Zhang; Donghua Wang; Ning Wang; Chang Liu; Ziyue Li; Liqi Wang; Xiuqing Zhu; Dianyu Yu

doi:10.1016/j.bios.2023.115751

Self-powered biosensor using photoactive ternary nanocomposite: Testing the phospholipid content in rhodotorula glutinis oil

Biosens Bioelectron. 2023 Dec 15:242:115751. doi: 10.1016/j.bios.2023.115751. Epub 2023 Oct 11.

Authors

Weining Wang¹, Hairong Zhang², Donghua Wang³, Ning Wang¹, Chang Liu¹, Ziyue Li⁴, Liqi Wang⁵, Xiuqing Zhu⁶, Dianyu Yu⁴

Affiliations

¹ School of Food Engineering, Harbin University of Commerce, Harbin, 150028, China.
² School of Computer and Information Engineering, Harbin University of Commerce, Harbin, 150028, China.
³ School of Food Engineering, Harbin University of Commerce, Harbin, 150028, China; School of Food Science, Northeast Agricultural University, Harbin, 150030, China.
⁴ School of Food Science, Northeast Agricultural University, Harbin, 150030, China.
⁵ School of Food Engineering, Harbin University of Commerce, Harbin, 150028, China; School of Computer and Information Engineering, Harbin University of Commerce, Harbin, 150028, China. Electronic address: hsdwlq@163.com.
⁶ School of Food Engineering, Harbin University of Commerce, Harbin, 150028, China. Electronic address: xqzhuwang@163.com.

PMID: 37839349
DOI: 10.1016/j.bios.2023.115751

Abstract

In the field of oil refining, the presence of excessive residual phosphorus in crude oil can significantly impact its quality, thereby emphasizing the necessity for compact and convenient testing equipment. This study primarily focuses on developing of self-powered biosensor (SPB) using immobilizing Choline Oxidase with a photoactive ternary nanocomposite complex (CHOx-BiOI-rGO-Fe₃O₄ NPs-ITO) as the anode and utilizing a Pt electrode as the cathode. The successful preparation of the ternary composite photoelectrode for the anode was confirmed through a range of characterization techniques, including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM), Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), N₂ absorption/desorption, Dynamic light scattering (DLS), and Ultraviolet-visible diffuse reflection spectrometer (UV-vis DRS). The electrochemical and photoelectrochemical properties were assessed using an electrochemical workstation, revealing a significant enhancement photoelectrical responsiveness attributed to the formation of heterojunction structures. The SPB exhibited a remarkable linear relationship between the instantaneous photocurrent and phosphatidylcholine (PC) concentration, with a regression equation of I (μA) = 39.62071C (mM) + 3.47271. The linear range covered a concentration range of 0.01-10 mM, and the detection limit (S/N = 3) was determined to be 0.008 mM. It demonstrated excellent reproducibility and storage stability, positioning it a promising alternative to High-performance liquid chromatography (HPLC) for accurate quantification of PC content in rhodotorula glutinis oil. The standard recovery PC content ranged from 98.48% to 103.53%, with a relative standard deviation (RSD) ranging from 1.4% to 2.4%. This research presents a convenient and precise detection device that has the potential to address the issue of lagging detection in the oil refining process.

Keywords: Lecithin; Oil refining; Photochemistry; Rapid detection; Self-powered biosensor.

Published by Elsevier B.V.

MeSH terms

Biosensing Techniques* / methods
Nanocomposites* / chemistry
Phospholipids
Reproducibility of Results

Substances

Phospholipids

Supplementary concepts

Rhodotorula glutinis