Physiological and transcriptomic analysis of the mangrove species Kandelia obovata in response to flooding stress

Shuangshuang Liu; Sheng Yang; Huizi Liu; Qingdi Hu; Xing Liu; Jinwang Wang; Jiayu Wang; Wenzhen Xin; Qiuxia Chen

doi:10.1016/j.marpolbul.2023.115598

Physiological and transcriptomic analysis of the mangrove species Kandelia obovata in response to flooding stress

Mar Pollut Bull. 2023 Nov:196:115598. doi: 10.1016/j.marpolbul.2023.115598. Epub 2023 Oct 13.

Authors

Shuangshuang Liu¹, Sheng Yang², Huizi Liu², Qingdi Hu², Xing Liu², Jinwang Wang², Jiayu Wang³, Wenzhen Xin³, Qiuxia Chen⁴

Affiliations

¹ Zhejiang Institute of Subtropical Crops, Zhejiang Academy of Agricultural Sciences, Wenzhou 325005, China; College of Forestry and Biotechnology, Zhejiang Agricultural and Forestry University, Hangzhou 311300, China.
² Zhejiang Institute of Subtropical Crops, Zhejiang Academy of Agricultural Sciences, Wenzhou 325005, China.
³ Zhejiang Institute of Subtropical Crops, Zhejiang Academy of Agricultural Sciences, Wenzhou 325005, China; College of Life and Environmental Sciences, Wenzhou University, Wenzhou 325035, China.
⁴ Zhejiang Institute of Subtropical Crops, Zhejiang Academy of Agricultural Sciences, Wenzhou 325005, China. Electronic address: yzscqx@163.com.

PMID: 37839131
DOI: 10.1016/j.marpolbul.2023.115598

Abstract

Flooding stress on mangroves is growing continually with rising sea level. In this study, the physiology and transcriptome of the mangrove species Kandelia obovata under flooding stress were analyzed. With increasing inundation time, malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), soluble sugar (SS), soluble protein (SP), and proline (Pro) content declined, while peroxidase (POD) and ascorbate peroxidase (APX) activity rose significantly. According to the KEGG pathway enrichment analysis, upregulated differentially expressed genes (DEGs) were enriched in the plant hormone signaling pathway. Furthermore, MYB44 and MYB108 genes from the MYB transcription factor family and RAP2.12, DREB2B, and ERF4 genes from the AP2/ERF family were up-regulated under flooding conditions. A strong correlation was established between the expression levels of 12 DEGs under flooding stress and RNA sequencing data and was verified by qRT-PCR. These results provide new insights into the molecular mechanism of K. obovata in response to flooding stress.

Keywords: Flooding stress; Physiological analysis; RNA-seq; Transcriptional regulation.

MeSH terms

Antioxidants / metabolism
Gene Expression Profiling
Glutathione / metabolism
Rhizophoraceae* / metabolism
Stress, Physiological
Superoxide Dismutase / metabolism
Transcriptome

Substances

Antioxidants
Superoxide Dismutase
Glutathione