Comparison effect of collagen/P3HB composite scaffold and human amniotic membrane loaded with mesenchymal stem cells on colon anastomosis healing in male rats

Mozhdeh Zamani; Ali Zahedian; Nader Tanideh; Zahra Khodabandeh; Farhad Koohpeyma; Hajar Khazraei; Shahrokh Zare; Moein Zarei; Seyed Vahid Hosseini

doi:10.1016/j.bbrc.2023.10.020

Comparison effect of collagen/P3HB composite scaffold and human amniotic membrane loaded with mesenchymal stem cells on colon anastomosis healing in male rats

Biochem Biophys Res Commun. 2023 Nov 19:682:281-292. doi: 10.1016/j.bbrc.2023.10.020. Epub 2023 Oct 5.

Authors

Mozhdeh Zamani¹, Ali Zahedian², Nader Tanideh³, Zahra Khodabandeh³, Farhad Koohpeyma⁴, Hajar Khazraei², Shahrokh Zare³, Moein Zarei⁵, Seyed Vahid Hosseini⁶

Affiliations

¹ Autophagy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
² Colorectal Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
³ Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
⁴ Endocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
⁵ Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; West Pomeranian University of Technology, Szczecin, Department of Polymer and Biomaterials Science, Al. Piastow 45, 71-311, Szczecin, Poland. Electronic address: zm47832@zut.edu.pl.
⁶ Colorectal Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address: hoseiniv@sums.ac.ir.

PMID: 37832385
DOI: 10.1016/j.bbrc.2023.10.020

Abstract

Covering surgical wounds with biomaterials, biologic scaffolds, and mesenchymal stem cells (MSCs) improves the healing process and reduces postoperative complications. This study was designed to evaluate and compare the effect of MSC-free/MSC-seeded new collagen/poly(3-hydroxybutyrate) (COL/P3HB) composite scaffold and human amniotic membrane (HAM) on the colon anastomosis healing process. COL/P3HB scaffold was prepared using freeze-drying method. MSCs were isolated and characterized from rat adipose tissue. After biocompatibility evaluation by MTT assay, MSCs were seeded on the scaffold and HAM by micro-mass seeding technique. In total, 35 male rats were randomly divided into five groups. After the surgical procedure, cecum incisions were covered by the MSC-free/MSC-seeded scaffold or HAM. Incisions in the control group were only sutured. One month later, the healing process was determined by stereological analysis. The Kruskal-Wallis followed by Dunn's tests were utilized for statistical outcome analysis (SPSS software version 21). COL/10% P3HB scaffold showed the best mechanical and structural properties (7.86 MPa strength, porosity more than 75%). MTT assay indicated that scaffold and especially HAM have suitable biocompatibility. Collagenization and neovascularization were significantly higher, and necrosis was considerably lower in all treated groups in comparison with the controls. MSC-seeded scaffold and HAM significantly decrease inflammation and increase gland volume compared with other groups. The MSC-seeded HAM was significantly successful in decreasing edema compared with other groups. Newly synthesized COL/P3HB scaffold improves the colon anastomosis healing; however, the major positive effect belonged to HAM. MSCs remarkably increase their healing process. Further investigations may contribute to confirming these results in other wound healing.

Keywords: Collagen; Composite scaffold; Healing process; Human amniotic membrane; Mesenchymal stem cells; Poly(3-hydroxybutyrate).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amnion
Anastomosis, Surgical
Animals
Collagen / chemistry
Colon / surgery
Humans
Male
Mesenchymal Stem Cells*
Rats
Tissue Scaffolds* / chemistry
Wound Healing

Substances

Collagen