Semiconducting single-walled carbon nanotubes (s-SWCNT) are structures that fluoresce in the near-infrared region. By coating SWCNT surfaces with polymeric materials such as single-chain DNA, changes in fluorescence emission occur in the presence of reagents. In this way, polymer-coated SWCNT structures allow them to be used as optical sensors for single molecule detection. Especially today, the inadequacy of the methods used in the detection of cellular molecules makes the early diagnosis of diseases such as cancer difficult at the single-molecule level. In this study, the detection of nitric oxide (NO) signals, which are a marker of cancer, was carried out at the single-molecule level. In this context, a sensor structure was formed by coating the 7,6-chiral s-SWCNT surface with ssDNA with different oligonucleotide lengths (AT). The sensor structure was characterized by using UV-vis spectroscopy and Raman spectroscopy microscopy. After formation of the sensor structure, a selectivity library was created using various molecules. As a result of the coating of the SWCNT (7,6) surface with DNA corona phase formation, Raman peaks at 195 and 276 cm-1 were observed to shift to the right. Additionally, the selectivity library results showed that the (AT)30 sequence can be used in NO detection. As a result of the studies using SWCNT (7.6)- (AT)30, the limit of detection (LOD) and limit of determination (LOQ) values of the sensor against NO were found to be 1.24 and 4.13 μM, respectively.
Keywords: bosensor; fluorescence; nitric oxide; single-walled carbon nanotube; ss-DNA.