Enhanced antimicrobial properties and bioactivity of 3D-printed titanium scaffolds by multilayer bioceramic coating for large bone defects

Marija Milivojevic; Ke Chen; Zeljko Radovanovic; Rada Petrovic; Suzana Dimitrijevic-Brankovic; Vesna Kojic; Danica Markovic; Djordje Janackovic

doi:10.1088/1748-605X/ad02d2

Enhanced antimicrobial properties and bioactivity of 3D-printed titanium scaffolds by multilayer bioceramic coating for large bone defects

Biomed Mater. 2023 Oct 26;18(6). doi: 10.1088/1748-605X/ad02d2.

Authors

Marija Milivojevic¹, Ke Chen², Zeljko Radovanovic¹, Rada Petrovic³, Suzana Dimitrijevic-Brankovic³, Vesna Kojic⁴, Danica Markovic⁵, Djordje Janackovic³

Affiliations

¹ Innovation Center of the Faculty of Technology and Metallurgy in Belgrade Ltd, Belgrade, Serbia.
² School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
³ Faculty of Technology and Metallurgy, University of Belgrade, Belgrade, Serbia.
⁴ Faculty of Medicine, Oncology Institute of Vojvodina, University of Novi Sad, Sremska Kamenica, Serbia.
⁵ Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia.

PMID: 37827161
DOI: 10.1088/1748-605X/ad02d2

Abstract

The restoration of large bone defects caused by trauma, tumor resection, or infection is a major clinical problem in orthopedics and dentistry because postoperative infections, corrosion, and limited osteointegration of metal implants can lead to loosening of the implant. The aim of this study was to improve the surface properties of a 3D-printed (electron beam melting) Ti6Al4V-based macroporous scaffold by multilayer coating with bioactive silicate glasses (BAGs) and hydroxyapatite doped with a silver (AgHAP) or AgHAP additionally sonochemically modified with ZnO (ZnO-AgHAP). The coated scaffolds AgHAP_BAGs_Ti and ZnO-AgHAP_BAGs_Ti enhanced cytocompatibility in L929 and MRC5 cell lines and expressed bioactivity in simulated body fluid. A lower release of vanadium ions in coated samples compared to bare Ti scaffold indicates decreased dissolution of Ti alloy in coated samples. The coated samples reduced growth ofEscherichia coliandStaphylococcus aureusfor 4-6 orders of magnitude. Therefore, the 3D-printed Ti-based scaffolds coated with BAGs and (ZnO-)AgHAP have great potential for application as a multifunctional implant with antibacterial properties for the restoration of defects in load-bearing bones.

Keywords: antibacterial coatings; electron beam melting fabrication; graded bioactive glasses; hydroxyapatite; large bone defect; sonochemical synthesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / chemistry
Coated Materials, Biocompatible* / chemistry
Printing, Three-Dimensional
Surface Properties
Titanium / chemistry
Zinc Oxide*

Substances

Coated Materials, Biocompatible
Zinc Oxide
Titanium
Anti-Bacterial Agents