Development and characterization of rice bran-gum Arabic based encapsulated biofertilizer for enhanced shelf life and controlled bacterial release

Rajinder Kaur; Sukhminderjit Kaur; Vagish Dwibedi; Charanjit Kaur; Nadeem Akhtar; Abdulhakeem Alzahrani

doi:10.3389/fmicb.2023.1267730

Development and characterization of rice bran-gum Arabic based encapsulated biofertilizer for enhanced shelf life and controlled bacterial release

Front Microbiol. 2023 Sep 19:14:1267730. doi: 10.3389/fmicb.2023.1267730. eCollection 2023.

Authors

Rajinder Kaur¹, Sukhminderjit Kaur¹, Vagish Dwibedi¹, Charanjit Kaur², Nadeem Akhtar³, Abdulhakeem Alzahrani⁴

Affiliations

¹ Department of Biotechnology, University Institute of Biotechnology, Chandigarh University, Mohali, India.
² Department of Microbiology, Bhojia Institute of Life Sciences, Baddi, India.
³ Department of Animal Biosciences, University of Guelph, Guelph, ON, Canada.
⁴ Department of Food Science & Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia.

Abstract

Introduction: Currently, microbe-based approaches are being tested to address nutrient deficiencies and enhance nutrient use efficiency in crops. However, these bioinoculants have been unsuccessful at the commercial level due to differences in field and in-vivo conditions. Thus, to enhance bacterial stability, microbial formulations are considered, which will provide an appropriate microenvironment and protection to the bacteria ensuring better rhizospheric-colonization.

Methods: The present study aimed to develop a phosphobacterium-based encapsulated biofertilizer using the ion-chelation method, wherein a bacterial strain, Myroid gitamensis was mixed with a composite solution containing rice bran (RB), gum Arabic (GA), tricalcium phosphate, and alginate to develop low-cost and slow-release microbeads. The developed microbead was studied for encapsulation efficiency, shape, size, external morphology, shelf-life, soil release behavior, and biodegradability and characterized using SEM, FTIR, and XRD. Further, the wheat growth-promoting potential of microbeads was studied.

Results: The developed microbeads showed an encapsulation efficiency of 94.11%. The air-dried beads stored at 4°C were favorable for bacterial survival for upto 6 months. Microbeads showed 99.75% degradation within 110 days of incubation showing the bio-sustainable nature of the beads. The application of dried formulations to the pot-grown wheat seedlings resulted in a higher germination rate, shoot length, root length, fresh weight, dry weight of the seedlings, and higher potassium and phosphorus uptake in wheat.

Discussion: This study, for the first time, provides evidence that compared to liquid biofertilizers, the RB-GA encapsulated bacteria have better potential of enhancing wheat growth and can be foreseen as a future fertilizer option for wheat.

Keywords: Myroid gitamensis; biofertilizer; carrier-based biofertilizers; encapsulation; liquid biofertilizer; plant growth promotion.