T7 expression plasmids for producing a recombinant human G1P[8] rotavirus comprising RIX4414 sequences of the RV1 (Rotarix , GSK) vaccine strain

Asha A Philip; Chantal A Agbemabiese; Guanghui Yi; John T Patton

doi:10.1128/MRA.00603-23

T7 expression plasmids for producing a recombinant human G1P[8] rotavirus comprising RIX4414 sequences of the RV1 (Rotarix , GSK) vaccine strain

Microbiol Resour Announc. 2023 Nov 16;12(11):e0060323. doi: 10.1128/MRA.00603-23. Epub 2023 Oct 11.

Authors

Asha A Philip¹, Chantal A Agbemabiese², Guanghui Yi¹, John T Patton¹

Affiliations

¹ Department of Biology, Indiana University Bloomington , Bloomington, Indiana, USA.
² Department of Electron Microscopy and Histopathology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana , Accra, Ghana.

Abstract

The live oral rotavirus RV1 (Rotarix) vaccine is formulated from the human G1P[8] RIX4414 virus. Based on RIX4414 sequences, T7 expression plasmids were constructed that supported recovery of recombinant RIX4414-like viruses by reverse genetics. These plasmids will advance the study of the RV1 vaccine, possibly allowing improvements to its efficacy.

Keywords: RIX4414 virus; recombinant virus; reverse genetic analysis; rotavirus; vaccines.

Grants and funding

R21 AI144881/AI/NIAID NIH HHS/United States