A specific and rapid method for detecting Bacillus and Acinetobacter species in Daqu

Yanwei Wei; Shuyue Zhang; Guikun Guan; Ziran Wan; Ruiming Wang; Piwu Li; Yu Liu; Junqing Wang; Guanhua Jiao; Hao Wang; Chuying Sun

doi:10.3389/fbioe.2023.1261563

A specific and rapid method for detecting Bacillus and Acinetobacter species in Daqu

Front Bioeng Biotechnol. 2023 Sep 25:11:1261563. doi: 10.3389/fbioe.2023.1261563. eCollection 2023.

Authors

Yanwei Wei^{1

2}, Shuyue Zhang^{1

2}, Guikun Guan³, Ziran Wan³, Ruiming Wang^{1

2}, Piwu Li^{1

2}, Yu Liu³, Junqing Wang^{1

2}, Guanhua Jiao^{1

2}, Hao Wang^{1

2}, Chuying Sun^{1

2}

Affiliations

¹ State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology, Jinan, Shandong, China.
² Department of Biological Engineering, Qilu University of Technology, Jinan, Shandong, China.
³ Lanling Meijiu Co., Ltd., Lanling, Shandong, China.

Abstract

Daqu is a spontaneous, solid-state cereal fermentation product used for saccharification and as a starter culture for Chinese Baijiu production. Bacillus and Acinetobacter, two dominant microbial genera in Daqu, produce enzymes and organic acids that influence the Daqu quality. However, there are no rapid analytical methods for detecting Bacillus and Acinetobacter. We designed primers specific to the genera Bacillus and Acinetobacter to perform genetic comparisons using the 16 S rRNA. After amplification of polymerase chain reaction using specific primers, high-throughput sequencing was performed to detect strains of Bacillus and Acinetobacter. The results showed that the effective amplification rates for Bacillus and Acinetobacter in Daqu were 86.92% and 79.75%, respectively. Thus, we have devised and assessed a method to accurately identify the species associated with Bacillus and Acinetobacter in Daqu, which can also hold significance for bacterial typing and identification.

Keywords: Acinetobacter; Bacillus; Baijiu production; Daqu; high-throughput sequencing; specific primer PCR.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by the Focus on Research and Development Plan in Shandong Province (2021ZDSYS10, 2022CXGC020206), Taishan Scholar Foundation of Shandong Province (tscx202306067), Innovation Fund for Small and Medium-sized Technology Innovation Capacity Enhancement Project of Shandong Province (2023TS1047), Key innovation Project of Qilu University of Technology (Shandong Academy of Sciences) (2022JBZ01-06), and National Natural Science Foundation of China (31801527).