Effect of Streptomyces JD211 application on soil physicochemical properties and N2O emission characteristics of rice rhizosphere

Hailong Peng; Tianyu Xu; Lixin Wang; Jiaqing Yu; Xin Chen; Xin Cheng; Hanguang Li; Lin Huang; Lei Wei; Saijin Wei

doi:10.1016/j.scitotenv.2023.167673

Effect of Streptomyces JD211 application on soil physicochemical properties and N₂O emission characteristics of rice rhizosphere

Sci Total Environ. 2024 Jan 1:906:167673. doi: 10.1016/j.scitotenv.2023.167673. Epub 2023 Oct 7.

Authors

Hailong Peng¹, Tianyu Xu¹, Lixin Wang¹, Jiaqing Yu¹, Xin Chen¹, Xin Cheng¹, Hanguang Li¹, Lin Huang¹, Lei Wei², Saijin Wei³

Affiliations

¹ College of Biological Science and Engineering, Jiangxi Collaborative Innovation Center of Modernization production of Double-cropping Rice, Jiangxi Provincial Key Lab of Crop Physiology, Ecology and Genetic Breeding, Jiangxi Provincial Engineering Lab for Development and Utilization of Agricultural Microbial resources, Institute of Applied Microbiology, Jiangxi Agricultural University, Nanchang, China.
² College of Biological Science and Engineering, Jiangxi Collaborative Innovation Center of Modernization production of Double-cropping Rice, Jiangxi Provincial Key Lab of Crop Physiology, Ecology and Genetic Breeding, Jiangxi Provincial Engineering Lab for Development and Utilization of Agricultural Microbial resources, Institute of Applied Microbiology, Jiangxi Agricultural University, Nanchang, China. Electronic address: 13928750620@163.com.
³ College of Biological Science and Engineering, Jiangxi Collaborative Innovation Center of Modernization production of Double-cropping Rice, Jiangxi Provincial Key Lab of Crop Physiology, Ecology and Genetic Breeding, Jiangxi Provincial Engineering Lab for Development and Utilization of Agricultural Microbial resources, Institute of Applied Microbiology, Jiangxi Agricultural University, Nanchang, China. Electronic address: weisaijin@126.com.

PMID: 37813263
DOI: 10.1016/j.scitotenv.2023.167673

Abstract

Biocontrol agent, as a pollution-free and sustainable plant disease control method, can inhibit the spread of soil-borne diseases and promote the growth of crops. However, there are few studies on the effect of biocontrol agent on N₂O emission in rice soil. In this study, after the application of the biocontrol agent Streptomyces JD211, N₂O emission from rice soil were measured, and the relationship between the agent and soil N₂O emissions were studied in soil chemistry and molecular biology. The results showed that the application of Streptomyces JD211 can significantly reduce the rate of N₂O emission from rice soil. The NH₄⁺-N and NO₃^--N contents in rice soil decreased in a short period of time after the application of Streptomyces JD211, while the mineral N content in the soil remained stable with rice growth. 16S rRNA gene sequencing and metagenomic sequencing revealed Streptomyces JD211 application mainly increased the relative abundance of Burkholderia and Streptomyces in the soil microbial community, reduced the relative abundance of hao, norB, norC genes, and increased the relative abundance of nosZ and hcp genes. Streptomyces JD211 application promoted N₂O transformation and weakened N₂O production pathways, which ultimately reduced N₂O emissions from rice soils. This study provided new insight of biocontrol agents to regulate soil N₂O emissions, which is of great significance for the development and application of biocontrol bacteria and farmland environmental protection.

Keywords: Biocontrol agent; Functional gene; N(2)O emission; Rice; Streptomyces JD211.

MeSH terms

Nitrous Oxide / analysis
Oryza*
RNA, Ribosomal, 16S
Rhizosphere
Soil / chemistry
Soil Microbiology
Streptomyces*

Substances

Soil
RNA, Ribosomal, 16S
Nitrous Oxide