Current research status of tumor cell biomarker detection

Liying Jiang; Xinyi Lin; Fenghua Chen; Xiaoyun Qin; Yanxia Yan; Linjiao Ren; Hongyu Yu; Lingqian Chang; Yang Wang

doi:10.1038/s41378-023-00581-5

Current research status of tumor cell biomarker detection

Microsyst Nanoeng. 2023 Oct 5:9:123. doi: 10.1038/s41378-023-00581-5. eCollection 2023.

Authors

Liying Jiang^{1

2}, Xinyi Lin¹, Fenghua Chen¹, Xiaoyun Qin¹, Yanxia Yan¹, Linjiao Ren¹, Hongyu Yu³, Lingqian Chang^#⁴, Yang Wang^#^{4

5}

Affiliations

¹ School of Electrical and Information Engineering, Zhengzhou University of Light Industry, Zhengzhou, 450002 China.
² Academy for Quantum Science and Technology, Zhengzhou University of Light Industry, Zhengzhou, 450002 China.
³ Department of Mechanical and Aerospace Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China.
⁴ key Laboratory of Biomechanics and Mechanobiology (Beihang University), Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100083 China.
⁵ School of Engineering Medicine, Beihang University, Beijing, 100083 China.

^# Contributed equally.

Abstract

With the annual increases in the morbidity and mortality rates of tumors, the use of biomarkers for early diagnosis and real-time monitoring of tumor cells is of great importance. Biomarkers used for tumor cell detection in body fluids include circulating tumor cells, nucleic acids, protein markers, and extracellular vesicles. Among them, circulating tumor cells, circulating tumor DNA, and exosomes have high potential for the prediction, diagnosis, and prognosis of tumor diseases due to the large amount of valuable information on tumor characteristics and evolution; in addition, in situ monitoring of telomerase and miRNA in living cells has been the topic of extensive research to understand tumor development in real time. Various techniques, such as enzyme-linked immunosorbent assays, immunoblotting, and mass spectrometry, have been widely used for the detection of these markers. Among them, the detection of tumor cell markers in body fluids based on electrochemical biosensors and fluorescence signal analysis is highly preferred because of its high sensitivity, rapid detection and portable operation. Herein, we summarize recent research progress in the detection of tumor cell biomarkers in body fluids using electrochemical and fluorescence biosensors, outline the current research status of in situ fluorescence monitoring and the analysis of tumor markers in living cells, and discuss the technical challenges for their practical clinical application to provide a reference for the development of new tumor marker detection methods.

Keywords: Biosensors; Sensors.

Publication types

Review