miR-624 accelerates the growth of liver cancer cells by inhibiting EMC3

Xiaoxue Jiang; Yi Lu; Sijie Xie; Yingji Chen; Xinlei Liu; Shujie Li; Shuting Song; Liyan Wang; Dongdong Lu

doi:10.1016/j.ncrna.2023.09.005

miR-624 accelerates the growth of liver cancer cells by inhibiting EMC3

Noncoding RNA Res. 2023 Sep 24;8(4):641-644. doi: 10.1016/j.ncrna.2023.09.005. eCollection 2023 Dec.

Authors

Xiaoxue Jiang¹, Yi Lu², Sijie Xie¹, Yingji Chen¹, Xinlei Liu¹, Shujie Li¹, Shuting Song¹, Liyan Wang¹, Dongdong Lu¹

Affiliations

¹ Shanghai Putuo People's Hospital, School of Life Science and Technology, Tongji University, Shanghai, 200092, China.
² Departments of Geriatrics, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.

Abstract

miRNA is a noncoding RNA found in recent years and more than one third of human genes are the target of miRNAs. miR-624, located on human chromosome 14, is associated with tumorigenesis. However, the role of miR-624 in human hepatocarcinogenesis is still unclear. Herein, our results indicate that miR-624 accelerates the growth of liver cancer cells in vivo and in vitro. Moreover, the modification distribution of H3K9me1 on chromosomes is different between rLV group and rLV-miR-624 group. miR-624 affects epigenetic regulation of several genes in human liver cancer cells, such as RAB21, SMARCD3, MAPK6,PRRX1, ZFHX3, EMC3 (TMEM111). Furthermore, miR-624 affects transcriptome of some genes in liver cancer, including RAB21， UBE2N， PPP1CC，KPNA3， RAB7A，CPEB2,KLF4， MARK2， JUN， ARF6， TMEM39A. On the other hand, miR-624 affects proteome of several genes in liver cancer, such as, RBM5,PTK2, KDM2A,POLR2H, POLR2G,CDK6,KIF15,CUL2,FKBP2,ErbB-3,JUN, PKM2, CyclinE,PLK1, mTOR, PPARγ, Rab7A,ARAF, UPF3B ，PTEN, SUZ12, GADD45, H3.3, CUL5, ARF6,EMC3,ATG4B,ATG14,CALR. Interestingly, miR-624 affects the RAB7A interaction network in liver cancer cells, involving in CLTC，ITGB1，HNRNPU， DARS1， RPS16， CTPS1，H3-3B，JUN,MYH10， CUL5， CPSF7. Strikingly, excessive MEC3 abrogates the carcinogenic functions of miR-624. Importantly, our findings indicate that miR-624 affects some signaling pathway in liver cancer, including Wnt signaling pathway，Hippo signaling pathway，mTOR signaling pathway, Ras signaling pathway，MAPK signaling pathway，PI3K-Akt signaling pathway, erbB signaling pathway. These results provide a basis for the treatment of human liver cancer.

Keywords: EMC3; Liver cancer; Proteome; Proteomics; Transcriptome; Transcriptomics; miR-624.