Effect of phosphorus and sodium acetate on lipid accumulation from Ankistrodesmus sp. IFRPD 1061 in an open pond

Paninee Jarungkeerativimol; Afrasiab Khan Tareen; Imrana Niaz Sultan; Muhammad Waseem Khan; Pramuk Parakulsuksatid

doi:10.1016/j.heliyon.2023.e19778

Effect of phosphorus and sodium acetate on lipid accumulation from Ankistrodesmus sp. IFRPD 1061 in an open pond

Heliyon. 2023 Sep 1;9(9):e19778. doi: 10.1016/j.heliyon.2023.e19778. eCollection 2023 Sep.

Authors

Paninee Jarungkeerativimol¹, Afrasiab Khan Tareen^{1

2}, Imrana Niaz Sultan^{1

2}, Muhammad Waseem Khan², Pramuk Parakulsuksatid¹

Affiliations

¹ Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, 50 Ngam Wong Wan Rd, Ladyaow, Chatuchak, Bangkok, 10900, Thailand.
² Department of Biotechnology, Balochistan University of Information Technology Engineering and Management Sciences, Quetta, 87300, Balochistan, Pakistan.

Abstract

Ankistrodesmus sp, has been comprehensively studied for their potential in the production of biodiesel due to their biomass productivity and high lipid content. This study examined the biomass productivity, and concentration, lipid productivity, and concentration, and lipid contents of Ankistrodesmus sp. IFRPD 1061 under several phosphorus concentrations. The optimum conditions were attained at 0.12 g/L KH₂PO₄. The highest lipid content reached to 35.950 ± 4.253% (w/w) in 22 days cultivation. An open pond cultivation system was used with the addition of 10 mM sodium acetate on every fourth day (0, 4, 8 and 12) of cultivation and KH₂PO₄ on twelfth day of cultivation. The obtained biomass productivity and concentration, lipid productivity and concentration and lipid content were 0.709 ± 0.027 g/L, 48.304 ± 1.894 mg/L/day, 0.214 ± 0.004 g/L 14.550 ± 0.215 mg/L/day and 30.154 ± 1.627% (w/w) in 14 days of cultivation, respectively. The results exhibited that addition of 10 mM sodium acetate and KH₂PO₄ may enhance lipid accumulation within algae cells in an open pond cultivation system.

Keywords: Lipid productivity; Microalgae; Open pond; Phosphorus; Sodium acetate.