TaGPX1-D overexpression provides salinity and osmotic stress tolerance in Arabidopsis

Shivi Tyagi; Shumayla; Yashraaj Sharma; Madhu; Alok Sharma; Ashutosh Pandey; Kashmir Singh; Santosh Kumar Upadhyay

doi:10.1016/j.plantsci.2023.111881

TaGPX1-D overexpression provides salinity and osmotic stress tolerance in Arabidopsis

Plant Sci. 2023 Oct 6:337:111881. doi: 10.1016/j.plantsci.2023.111881. Online ahead of print.

Authors

Shivi Tyagi¹, Shumayla¹, Yashraaj Sharma², Madhu¹, Alok Sharma¹, Ashutosh Pandey³, Kashmir Singh⁴, Santosh Kumar Upadhyay⁵

Affiliations

¹ Department of Botany, Panjab University, Chandigarh 160014, India.
² Department of Botany, Panjab University, Chandigarh 160014, India; Department of Biotechnology, Panjab University, Chandigarh 160014, India.
³ National Institute of Plant Genome Research, New Delhi, India.
⁴ Department of Biotechnology, Panjab University, Chandigarh 160014, India.
⁵ Department of Botany, Panjab University, Chandigarh 160014, India. Electronic address: skupadhyay@pu.ac.in.

PMID: 37806453
DOI: 10.1016/j.plantsci.2023.111881

Abstract

Glutathione peroxidases (GPXs) are known to play an essential role in guarding cells against oxidative stress by catalyzing the reduction of hydrogen peroxide and organic hydroperoxides. The current study aims functional characterization of the TaGPX1-D gene of bread wheat (Triticum aestivum) for salinity and osmotic stress tolerance. To achieve this, we initially performed the spot assays of TaGPX1-D expressing yeast cells. The growth of recombinant TaGPX1-D expressing yeast cells was notably higher than the control cells under stress conditions. Later, we generated transgenic Arabidopsis plants expressing the TaGPX1-D gene and investigated their tolerance to various stress conditions. The transgenic plants exhibited improved tolerance to both salinity and osmotic stresses compared to the wild-type plants. The higher germination rates, increased antioxidant enzymes activities, improved chlorophyll, carotenoid, proline and relative water contents, and reduced hydrogen peroxide and MDA levels in the transgenic lines supported the stress tolerance mechanism. Overall, this study demonstrated the role of TaGPX1-D in abiotic stress tolerance, and it can be used for improving the tolerance of crops to environmental stressors, such as salinity and osmotic stress in future research.

Keywords: Antioxidant; Bread wheat; Glutathione peroxidase; Osmotic; Salinity; Transgenic.