Primary dolomite is believed to be formed through cyanobacterial calcification, yet the details and mechanisms of this process are not fully understood. In this study, a freshwater filamentous cyanobacterium, Leptolyngbya boryana, was cultured and domesticated in artificial freshwater and brackish solutions with various Mg/Ca ratios. The hydrochemistry, the extracellular polymeric substance (EPS) composition, and precipitate mineralogy in the medium were monitored. The results showed that the L. boryana induced proto-dolomite precipitation in brackish medium with salinity of 1.5 % and Mg/Ca ratio of 5. The proto-dolomite in this study has a "double spherical" appearance and a hollow core, which may have originally been filled by the complex composed of EPS and amorphous CaMg carbonate. With regard to elemental composition, the cyanobacterially-induced proto-dolomite is rich in calcium inside and magnesium-rich on the surface, and cyanobacterial organic matter is sealed inside the particles during spherulite growth. In this study, the accelerators for Mg2+ to enter the carbonate lattice mainly include extracellular acidic amino acids and polysaccharides. The changes of these promoters among different cultures were related to the growth state of cyanobacteria under salinity stress. The polysaccharides concentration has a significant increasing in the dolomite-precipitating medium, indicating that it may be the main promoter of proto-dolomite precipitation and significantly increases the amount of Mg2+ precipitation. At the meantime, the amount of precipitated Ca2+ was suppressed by increasing salinity and Mg2+, thus leading to the precipitation of proto-dolomite in this shifting process. This study can potentially provide a reference for explaining the dolomite (proto-dolomite) precipitation in aerobic brackish environment where cyanobacteria thrive.
Keywords: EPS; Freshwater cyanobacteria; Polysaccharides; Protodolomite; Salinity.
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