A robust NIR fluorescence-activated probe for peroxynitrite imaging in cells and mice osteoarthritis models

Xiuyang Li; Xinrong Zou; Ping Xu; Meiling Pang; Lulu Zhao; Shihan Chen; Yongbo Peng; Sijing Liang; Zhongliang Deng

doi:10.1016/j.ab.2023.115338

A robust NIR fluorescence-activated probe for peroxynitrite imaging in cells and mice osteoarthritis models

Anal Biochem. 2023 Dec 1:682:115338. doi: 10.1016/j.ab.2023.115338. Epub 2023 Oct 5.

Authors

Xiuyang Li¹, Xinrong Zou², Ping Xu², Meiling Pang², Lulu Zhao², Shihan Chen², Yongbo Peng², Sijing Liang², Zhongliang Deng³

Affiliations

¹ Department of Orthopedics, Chongqing Geriatric Clinical Research Center, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, PR China; Department of Orthopedics and Osteoarthrosis, The Seventh People's Hospital of Chongqing, The Central Hospital Affiliated to Chongqing University of Technology, Chongqing, 400054, PR China. Electronic address: zhouxuedian@qq.com.
² College of Pharmacy, Chongqing Medical University, Chongqing, 400016, PR China.
³ Department of Orthopedics, Chongqing Geriatric Clinical Research Center, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, PR China. Electronic address: dengzl@cqmu.edu.cn.

PMID: 37802174
DOI: 10.1016/j.ab.2023.115338

Abstract

Osteoarthritis (OA) is the most common type of joint disease, which is difficult to treat, but early standardized diagnosis and treatment can effectively alleviate the pain and symptoms of patients. Therefore, it is important to construct an effective tool to assist in the early diagnosis and evaluation of the therapeutic effect of OA. In this work, a near-infrared (NIR) fluorescence-activated fluorescent probe, YB-1, was constructed for the evaluation of the diagnostic and therapeutic efficacy of OA via detection and imaging of the biomarker of ONOO^- in inflammatory cells and mice osteoarthritis models. YB-1 exhibited high selectivity, high sensitivity, and a high ratio yield (I₆₆₈/I₀) fluorescence increasing (∼30 folds). Besides, YB-1 can be used effectively to image endogenous and exogenous ONOO^- in living human chondrocytes cells (TC28a2), as well as to evaluate the effect of drug (Chrysosplenol D, CD) treatment in IL-1β-induced inflammatory cells model. Interestingly, YB-1 was available for OONO^- imaging analysis in the collagenase-induced mice OA models and assessment of the effect of CD treatment in mice OA models, with good results. Thus, the newly constructed YB-1 is a powerful molecular tool for the diagnosis and treatment of OA-related diseases.

Keywords: Human chondrocytes cells (TC28a2); Imaging analysis; Osteoarthritis (OA); Reactive oxygen (ROS).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chondrocytes
Diagnostic Imaging
Disease Models, Animal
Fluorescent Dyes* / pharmacology
Humans
Mice
Osteoarthritis* / diagnostic imaging
Peroxynitrous Acid / pharmacology
Peroxynitrous Acid / therapeutic use

Substances

Fluorescent Dyes
Peroxynitrous Acid