Synthesis of Silanized Bioactive Glass/Gelatin Methacrylate (GelMA/Si-BG) composite hydrogel for Bone Tissue Engineering Application

Hsuan Chen; Yuan-Min Lin; Sasinan Bupphathong; Joshua Lim; Jing-En Huang; Wei Huang; Tiffany Angela S Hsieh; Chih-Hsin Lin

doi:10.1016/j.jmbbm.2023.106159

Synthesis of Silanized Bioactive Glass/Gelatin Methacrylate (GelMA/Si-BG) composite hydrogel for Bone Tissue Engineering Application

J Mech Behav Biomed Mater. 2023 Nov:147:106159. doi: 10.1016/j.jmbbm.2023.106159. Epub 2023 Sep 30.

Authors

Hsuan Chen¹, Yuan-Min Lin², Sasinan Bupphathong³, Joshua Lim⁴, Jing-En Huang¹, Wei Huang⁵, Tiffany Angela S Hsieh⁶, Chih-Hsin Lin⁷

Affiliations

¹ Department of Dentistry, School of Dentistry, National Yang Ming Chiao Tung University, Taipei, 112304, Taiwan.
² Department of Dentistry, School of Dentistry, National Yang Ming Chiao Tung University, Taipei, 112304, Taiwan; Department of Stomatology, Taipei Veterans Hospital, Taipei, 11221, Taiwan; Institute of Oral Tissue Engineering and Biomaterials, National Yang Ming Chiao Tung University, Taipei, 112304, Taiwan.
³ Graduate Institute of Nanomedicine and Medical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei, 11031, Taiwan; High-value Biomaterials Research and Commercialization Center, National Taipei University of Technology, Taipei, 10608, Taiwan.
⁴ Graduate Institute of Nanomedicine and Medical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei, 11031, Taiwan.
⁵ Department of Orthodontics, Rutgers School of Dental Medicine, Newark, NJ, 07103, USA.
⁶ School of Biomedical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei, 11031, Taiwan.
⁷ Graduate Institute of Nanomedicine and Medical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei, 11031, Taiwan. Electronic address: melodylin@tmu.edu.tw.

PMID: 37797555
DOI: 10.1016/j.jmbbm.2023.106159

Abstract

Bioactive glass (BG) has been widely employed in the field of bone tissue engineering owing to its osteoconductive properties. These properties increase the stiffness and bioactivity of polymeric hydrogels, making them ideal for the repair, replacement, and regeneration of damaged bones. In this study, we investigated the effects of incorporating silanized 45S5 bioactive glass (Si-BG) into gelatin methacrylate (GelMA) hydrogel (GelMA/Si-BG) for potential bone tissue engineering. Our findings revealed that crosslinking GelMA with Si-BG had a striking increase in bioactivity with and without osteogenic induction of human mesenchymal stem cells (hMSCs) when compared to GelMA/BG hydrogels. Meanwhile, both GelMA/Si-BG and GelMA/BG hydrogels were able to maintain the cell viability of hMSC for up to 14 days. Additionally, GelMA/Si-BG hydrogels were shown to have a significantly higher compressive modulus than GelMA/BG hydrogels. This study has demonstrated the introduction of silanized 45S5 BG into GelMA hydrogel bioactivity and mechanical properties of GelMA hydrogels, exemplifying the potential application of silanization of BG in bone tissue engineering.

Keywords: Bioactive glass; Bone regeneration; GelMA; Mesenchymal stem cells; Silanization; Sol-gel.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bone and Bones
Gelatin*
Humans
Hydrogels / pharmacology
Methacrylates / pharmacology
Tissue Engineering*
Tissue Scaffolds

Substances

Gelatin
Hydrogels
Methacrylates