Proteomic response to phosphorus deficiency and aluminum stress of three aluminum-tolerant phosphobacteria isolated from acidic soils

Patricio Javier Barra; Paola Duran; Mabel Delgado; Sharon Viscardi; Stéphane Claverol; Giovanni Larama; Marc Dumont; María de la Luz Mora

doi:10.1016/j.isci.2023.107910

Proteomic response to phosphorus deficiency and aluminum stress of three aluminum-tolerant phosphobacteria isolated from acidic soils

iScience. 2023 Sep 14;26(10):107910. doi: 10.1016/j.isci.2023.107910. eCollection 2023 Oct 20.

Authors

Patricio Javier Barra^{1

2}, Paola Duran^{1

2

3}, Mabel Delgado¹, Sharon Viscardi⁴, Stéphane Claverol⁵, Giovanni Larama^{1

2}, Marc Dumont⁶, María de la Luz Mora¹

Affiliations

¹ Center of Plant, Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus, Universidad de La Frontera, Temuco 4811230, Chile.
² Biocontrol Research Laboratory, Universidad de La Frontera, Temuco 4811230, Chile.
³ Facultad de Ciencias Agropecuarias y Medioambiente, Departamento de Producción Agropecuaria, Universidad de La Frontera, Temuco 4811230, Chile.
⁴ Escuela de la Salud, Campus San Francisco, Universidad Católica de Temuco, Temuco 4811230, Chile.
⁵ Plateforme Protéome, Centre Génomique Fonctionnelle de Bordeaux, Université de Bordeaux, Bordeaux, France.
⁶ School of Biological Sciences, University of Southampton, Southampton, UK.

Abstract

Aluminum (Al)-tolerant phosphobacteria enhance plant growth in acidic soils by improving Al complexing and phosphorus (P) availability. However, the impact of Al stress and P deficiency on bacterial biochemistry and physiology remains unclear. We investigated the single and mutual effects of Al stress (10 mM) and P deficiency (0.05 mM) on the proteome of three aluminum-tolerant phosphobacteria: Enterobacter sp. 198, Enterobacter sp. RJAL6, and Klebsiella sp. RCJ4. Cultivated under varying conditions, P deficiency upregulated P metabolism proteins while Al exposure downregulated iron-sulfur and heme-containing proteins and upregulated iron acquisition proteins. This demonstrated that Al influence on iron homeostasis and bacterial central metabolism. This study offers crucial insights into bacterial behavior in acidic soils, benefiting the development of bioinoculants for crops facing Al toxicity and P deficiency. This investigation marks the first proteomic study on the interaction between high Al and P deficiency in acid soils-adapted bacteria.

Keywords: Bacteriology; Microbiology; proteomics; soil science.