Formation of hen egg white lysozyme derived amyloid-based hydrogels using different gelation agents: A potential tool for drug delivery

Abstract

Amyloids are highly stable protein fibrillar aggregates that get deposited in various parts of our body and cause detrimental diseases. But in nature, the presence of functional amyloids is also noted in bacteria that help them by forming hyphae, biofilm, protein reservoirs, signalling messengers, etc. Keeping this perspective in mind, the idea behind this research was to develop functional amyloids in the form of hydrogel and analyse its potential in the biomedical sector as a drug-delivery tool. The synthesis and characterisation of three types of amyloid-based hydrogels have been reported in this work. Hen Egg-White Lysozyme (HEWL) protein was chosen as the principal ingredient as it is extensively used as a standard protein for studying amyloidogenesis and has inherent antibacterial properties. Comparative studies of different hydrogel properties exhibited variations in the hydrogels based on compositional differences in them. Finally, a drug release assay was done on the synthesized hydrogels to explore their potential as drug delivery tools.

Keywords: Amyloid; Drug release; Gelatin; Hen egg-white lysozyme; Hydrogel; Polyethylene glycol.