Spraying phenolic acid-modifiedchitooligosaccharide derivatives improves anthocyanin accumulation in grape

Songpo Duan; Zhiming Li; Zaid Khan; Chunmei Yang; Bosi Lu; Hong Shen

doi:10.1016/j.fochx.2023.100770

Spraying phenolic acid-modifiedchitooligosaccharide derivatives improves anthocyanin accumulation in grape

Food Chem X. 2023 Jun 25:19:100770. doi: 10.1016/j.fochx.2023.100770. eCollection 2023 Oct 30.

Authors

Songpo Duan¹, Zhiming Li¹, Zaid Khan¹, Chunmei Yang¹, Bosi Lu¹, Hong Shen¹

Affiliation

¹ College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China.

Abstract

Herein, four chitooligosaccharide derivatives (COS-RA, COS-FA, COS-VA, COS-GA) were prepared by laccase-catalyzed chitooligosaccharide modification with rosmarinic acid (RA), ferulic acid (FA), gallic acid (GA), and vanillic acid (VA), and structures were characterized. RA and FA resulted in higher amino-substitution in the chitooligosaccharides than GA and VA. COS-RA and COS-FA had greater DPPH scavenging rates than COS-GA and COS-VA. Compared with COS treatment, spraying 250 mg L^-1 COS-RA or COS-VA 6 times (once per 7 days) increased soluble sugar and anthocyanin content by 18.6%-23.2% and 41.7%-46.7%, respectively, from the fruit expansion to harvest stage. COS-RA and COS-VA also enhanced gene expression related to anthocyanin synthesis (PAL, F3H, F3'5'H, DFR, and UFGT) and monomeric anthocyanin accumulation (Mal-3-O-glu, Petu-3-O-ace-glu, Del-3-O-glu). Therefore, chitooligosaccharide derivatives may improve grape fruit anthocyanin accumulation by regulating antioxidant systems, improving the photosynthetic rate and inducing gene expression related to anthocyanin synthesis.

Keywords: Anthocyanins; Antioxidant; Chitooligosaccharide derivatives; Grapes; Phenolic acid.