Analytical strategies based on untargeted and targeted metabolomics for the accurate authentication of organic milk from Jersey and Yak

Min Kang; Hongxia Wang; Chuxin Chen; Ran Suo; Jianfeng Sun; Quanhong Yue; Yaqiong Liu

doi:10.1016/j.fochx.2023.100786

Analytical strategies based on untargeted and targeted metabolomics for the accurate authentication of organic milk from Jersey and Yak

Food Chem X. 2023 Jul 8:19:100786. doi: 10.1016/j.fochx.2023.100786. eCollection 2023 Oct 30.

Authors

Min Kang¹, Hongxia Wang¹, Chuxin Chen¹, Ran Suo¹, Jianfeng Sun¹, Quanhong Yue¹, Yaqiong Liu¹

Affiliation

¹ College of Food Science and Technology, Hebei Agricultural University, 289th Lingyusi Street, Lianchi District, Baoding 071000, China.

Abstract

Organic milk has a high risk of food fraud as it can easily be adulterated with non-organic milk. This study aimed to identify metabolite markers for assessing the authenticity of organic milk from Jersey and Yak. In the untargeted strategy, ultra-high performance liquid chromatography-Q Exactive HF-X mass spectrometer coupled with chemometrics analysis was used to screen and identify tentative markers of organic milk from Jersey and Yak. In the targeted strategy, a quick and easy method of ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed to quantify three markers. The peptide of Thr-Ala-Val and D-biotin were determined to be metabolite markers for distinguishing organic and non-organic Jersey milk, whereas trimethylamine N-oxide was determined to be a metabolite marker for distinguishing organic and non-organic Yak milk. These findings provide critical information to facilitate assessments of organic milk authenticity.

Keywords: Authenticity; Jersey milk; Metabolomic; Yak milk.