Enhanced nitrogen removal in sewage treatment is achieved by using kitchen waste hydrolysate without a significant increase in nitrous oxide emissions

Abstract

Kitchen waste hydrolysate (KWH) is an effective replacement for commonly used carbon sources such as sodium acetate (NaAc) and glucose (Glu), in wastewater treatment plants (WWTPs) to enhance the total nitrogen (TN) removal efficiency in sewage and reduce the operating cost of WWTPs. However, KWH utilization introduces complex organic matter that may lead to increased nitrous oxide (N₂O) emissions, compared with that of NaAc and Glu, causing significant damage to the atmosphere. Therefore, this study aims to compare the effects of KWH, Glu, and NaAc on N₂O emissions in sewage treatment. The results indicated that KWH introduction did not lead to a significant increase in N₂O emissions, with a conversion rate of only 5.61 %. Compared with raw sludge, the addition of only Glu and NaAc significantly increased the abundance of the nar G gene, indicating that the readily degradable carbon sources initiated denitrification at a faster rate than KWH. When KWH was added, there was a notable increase in the abundance of genes associated with partial nitrification and denitrification (nir K, hzo, and nos Z). In contrast, Glu and NaAc did not have a significant effect on the nos Z gene. The results suggested that KWH supplementation was more effective to reduce N₂O to N₂. Moreover, the KWH addition significantly increased the microbial diversity in the sludge and promoted the presence of shortcut nitrification and denitrification bacteria (Comamonadaceae) and denitrification bacteria (Rhodobacteraceae), further indicating the potential of KWH for enhanced denitrification and reduced N₂O emissions. Overall, to the best of our knowledge, this is the first study that demonstrated KWH, as a novel and complex organic carbon source, can be safely used in sewage treatment processes to improve the pollutant removal efficiency without causing a significant increase in N₂O emissions.

Keywords: Bacterial community structure; Kitchen waste hydrolysate; N(2)O emission; Nitrogen transformation pathway; Nitrogen-cycling genes.