Construction of a synthetic microbial community based on multiomics linkage technology and analysis of the mechanism of lignocellulose degradation

Jinmeng Chen; Yafan Cai; Zhi Wang; Zhengzhong Xu; Jia Li; Xiaotian Ma; Wei Zhuang; Dong Liu; Shilei Wang; Andong Song; Jingliang Xu; Hanjie Ying

doi:10.1016/j.biortech.2023.129799

Construction of a synthetic microbial community based on multiomics linkage technology and analysis of the mechanism of lignocellulose degradation

Bioresour Technol. 2023 Dec:389:129799. doi: 10.1016/j.biortech.2023.129799. Epub 2023 Sep 27.

Authors

Jinmeng Chen¹, Yafan Cai¹, Zhi Wang¹, Zhengzhong Xu¹, Jia Li¹, Xiaotian Ma¹, Wei Zhuang², Dong Liu², Shilei Wang³, Andong Song⁴, Jingliang Xu¹, Hanjie Ying²

Affiliations

¹ School of Chemical Engineering, Zhengzhou University, 100 Ke xue Dadao, Zhengzhou 450001, China.
² School of Chemical Engineering, Zhengzhou University, 100 Ke xue Dadao, Zhengzhou 450001, China; National Engineering Technique Research Center for Biotechnology, State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 210009, China.
³ School of Chemical Engineering, Zhengzhou University, 100 Ke xue Dadao, Zhengzhou 450001, China. Electronic address: shileiwang@zzu.edu.cn.
⁴ College of Life Science, Henan Agricultural University, 218 Ping An Avenue, Zhengdong New District, Zhengzhou 450002, China.

PMID: 37774801
DOI: 10.1016/j.biortech.2023.129799

Abstract

The efficient degradation of lignocellulose is a bottleneck for its integrated utilization. This research performed species analysis and made functional predictions in various ecosystems using multiomics coupling to construct a core synthetic microbial community with efficient lignocellulose degradation function. The synthetic microbial community was employed to degrade corn straw via solid-state fermentation. The degradation mechanisms were resolved using proteomics. The optimum culture conditions included 10% inoculum level (w/v), 4% nitrogen source ratio and a fermentation time of 23 d. Under these conditions, the degradation rates of cellulose, hemicellulose, and lignin were 34.91%, 45.94%, and 23.34%, respectively. Proteomic analysis revealed that lignin 1,4-β-xylanase, β-xylosidase and endo-1,4-β-xylanase were closely related to lignocellulose degradation. The metabolic pathways involved in lignocellulose degradation and the functional roles of eight strains were obtained. The synthesis of a microbial community via multiomics linkage technology can effectively decompose lignocellulose, which is useful for their further utilization.

Keywords: Lignocellulose; Metagenomics; Proteomics; Solid-state fermentation; Synthetic microbial community.