Conductive PEDOT:PSS copolymer electrode coatings for selective detection of dopamine in ex vivo mouse brain slices

Dinakaran Thirumalai; Devaraju Subramani; Jaehoon Kim; Thenmozhi Rajarathinam; Jang-Hee Yoon; Hyun-Jong Paik; Jaewon Lee; Seung-Cheol Chang

doi:10.1016/j.talanta.2023.125252

Conductive PEDOT:PSS copolymer electrode coatings for selective detection of dopamine in ex vivo mouse brain slices

Talanta. 2024 Jan 15:267:125252. doi: 10.1016/j.talanta.2023.125252. Epub 2023 Sep 27.

Authors

Dinakaran Thirumalai¹, Devaraju Subramani², Jaehoon Kim³, Thenmozhi Rajarathinam⁴, Jang-Hee Yoon⁵, Hyun-Jong Paik⁶, Jaewon Lee⁷, Seung-Cheol Chang⁸

Affiliations

¹ BIT Convergence-based Innovative Drug Development Targeting Meta-inflammation, Pusan National University, Busan, 46241, Republic of Korea.
² Department of Polymer Science and Engineering, Pusan National University, Busan, 46241, Republic of Korea; Polymer Composites Lab, Department of Chemistry, School of Applied Science and Technology, Vignan's Foundation for Science, Technology, and Research (Deemed to be University), Vadlamudi, Guntur, Andhra Pradesh, 522213, India.
³ Department of Pharmacy, College of Pharmacy, Pusan National University, Busan, 46241, Republic of Korea.
⁴ Department of Cogno-Mechatronics Engineering, College of Nanoscience and Nanotechnology, Pusan National University, Busan, 46241, Republic of Korea.
⁵ Busan Centre, Korea Basic Science Institute, Busan, 46742, Republic of Korea.
⁶ Department of Polymer Science and Engineering, Pusan National University, Busan, 46241, Republic of Korea. Electronic address: hpaik@pusan.ac.kr.
⁷ BIT Convergence-based Innovative Drug Development Targeting Meta-inflammation, Pusan National University, Busan, 46241, Republic of Korea; Department of Pharmacy, College of Pharmacy, Pusan National University, Busan, 46241, Republic of Korea. Electronic address: neuron@pusan.ac.kr.
⁸ Department of Cogno-Mechatronics Engineering, College of Nanoscience and Nanotechnology, Pusan National University, Busan, 46241, Republic of Korea. Electronic address: s.c.chang@pusan.ac.kr.

PMID: 37774451
DOI: 10.1016/j.talanta.2023.125252

Abstract

A novel voltammetric sensor was developed to selectively determine dopamine (DA) concentration in the presence of ascorbic acid (AA) and 3,4-dihydroxyphenylacetic acid (DOPAC). This sensor utilizes a modified pencil graphite electrode (PGE) coated with a newly synthesized poly (3,4-ethylene dioxythiophene) (PEDOT):poly (styrene sulfonate-co-2-(3-(6-Methyl-4-oxo-1,4-dihydropyrimidin-2-yl) ureido) ethyl methacrylate) (P(SS-co-UPyMA)) composite. The PEDOT:P(SS-co-UPyMA) (PPU) composite was characterized using nuclear magnetic resonance, X-ray photoelectron, and Raman spectroscopies. The PPU-coated PGE was characterized using electrochemical techniques, including cyclic and differential pulse voltammetry. Compared to uncoated, PPU-coated PGE demonstrated improved sensitivity and selectivity for DA. The sensor exhibited a dynamic linear range of 0.1-300 μM for DA, with a detection limit of 44.4 nM (S/N = 3). Additionally, the PPU-coated PGE showed high reproducibility and storage stability for four weeks. To demonstrate its practical applicability, the PPU-coated PGE sensor was used for ex vivo brain slice samples from control and Parkinson's disease model mice.

Keywords: Copolymer; Disposable electrode; Dopamine; Electrode coating; Parkinson's disease; ex vivo brain slice.

MeSH terms

Animals
Ascorbic Acid / chemistry
Dopamine
Electrochemical Techniques / methods
Electrodes
Graphite* / chemistry
Mice
Reproducibility of Results

Substances

Graphite
poly(3,4-ethylene dioxythiophene)
Dopamine
Ascorbic Acid