Morchella esculenta cultivation in fallow paddy fields and drylands affects the diversity of soil bacteria and soil chemical properties

Mingzheng Duan; Chengcui Yang; Liuyuan Bao; Duo Han; Huaizheng Wang; Yongzhi Zhang; Honggao Liu; Shunqiang Yang

doi:10.3389/fgene.2023.1251695

Morchella esculenta cultivation in fallow paddy fields and drylands affects the diversity of soil bacteria and soil chemical properties

Front Genet. 2023 Sep 12:14:1251695. doi: 10.3389/fgene.2023.1251695. eCollection 2023.

Authors

Mingzheng Duan^#^{1

2}, Chengcui Yang^#^{1

2}, Liuyuan Bao^{1

2}, Duo Han^{1

2}, Huaizheng Wang^{1

2}, Yongzhi Zhang^{1

2}, Honggao Liu^{1

2}, Shunqiang Yang^{1

2}

Affiliations

¹ Yunnan Key Laboratory of Gastrodia Elata and Fungal Symbiotic Biology, College of Agronomy and Life Sciences, Zhaotong University, Zhaotong, China.
² Yunnan Engineering Research Center of Green Planting and Processing of Gastrodia Elata, College of Agronomy and Life Sciences, Zhaotong University, Zhaotong, China.

^# Contributed equally.

Abstract

The properties of paddy field (DT) and dry land (HD) soil and food production can be enhanced by the cultivation of Morchella esculenta (ME) during the fallow period. However, whether ME cultivation affects the soil health and microbial diversity of paddy fields and drylands during the cultivation period remains unclear, and this has greatly limited the wider use of this cultivation model. Here, we analyzed the soil chemical properties and bacterial diversity (via metabarcoding sequencing) of DT and HD soils following ME cultivation. Our findings indicated that ME cultivation could enhance soil health. The content of soil phosphorus and potassium (K) was increased in DT soil under ME cultivation, and the K content was significantly higher in HD soil than in DT soil under ME cultivation. ME cultivation had a weak effect on alpha diversity, and ME cultivation affected the abundance of some genera of soil bacteria. The cultivation of ME might reduce the methane production capacity of DT soil and enhance the nitrogen cycling process of HD soil based on the results of functional annotation analysis. Network analysis and correlation analysis showed that Gemmatimonas, Bryobacter, and Anaeromyxobacter were the key bacterial genera regulating soil chemical properties in DT soil under ME cultivation, and Bryobacter, Bacillus, Streptomyces, and Paenarthrobacter were the key taxa associated with the accumulation of K in HD soil. The results of our study will aid future efforts to further improve this cultivation model.

Keywords: 16S rRNA; NPK; corn; metabarcoding; rice; soils.

Grants and funding

This work was jointly funded by the project of “Kunpeng Plan” in Zhaotong City—Shunqiang Yang [Grant number 20211124]; the project of “Scientific and technological innovation team of agricultural waste resource utilization in universities of Yunnan Province” [Grant number 20190703]; project of “Yunnan Province expert workstation—Yucheng Dai” [Grant number 20211230].