Photoluminescence detection of nadifloxacin: Adopts switching off photoinduced electron transfer (PET)

Abobakr A Mohamed; Mahmoud A Omar; Sayed M Derayea; Mohamed Ahmed Elsayed; Islam M Mostafa

doi:10.1016/j.saa.2023.123451

Photoluminescence detection of nadifloxacin: Adopts switching off photoinduced electron transfer (PET)

Spectrochim Acta A Mol Biomol Spectrosc. 2024 Jan 15:305:123451. doi: 10.1016/j.saa.2023.123451. Epub 2023 Sep 23.

Authors

Abobakr A Mohamed¹, Mahmoud A Omar², Sayed M Derayea³, Mohamed Ahmed Elsayed¹, Islam M Mostafa⁴

Affiliations

¹ Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Fayoum University, 63514, Egypt.
² Department of Pharmacognosy and Pharmaceutical Chemistry, College of Pharmacy, Taibah University, Medinah 42210, Saudi Arabia; Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia 61511, Egypt.
³ Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia 61511, Egypt.
⁴ Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia 61511, Egypt; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China; University of Science and Technology of China, Hefei 230000, PR China. Electronic address: dr-islam@minia.edu.eg.

PMID: 37769466
DOI: 10.1016/j.saa.2023.123451

Abstract

A new fluorimetric sensing method for the selective and sensitive quantification of nadifloxacin (antibacterial agent, NDF) was established for the first time. The introduced method relies on blocking the photoinduced electron transfer (PET) effect of the nitrogen atom presented on the piperidine ring in NDF by fine-tuning the pH of the surrounding medium through its protonation using 0.5 M acetic acid. This protonation turns the weak fluorescence of NDF into stronger fluorescence intensity, allowing its selective and sensitive detection in its pure state as well as cream pharmaceutical formulation without any obstruction from the common excipients. The developed method has shown an NDF detection limit of 1.72 ng mL^-1 and a quantification limit of 5.20 ng mL^-1.

Keywords: Cream; Nadifloxacin; Pharmaceutical formulation; Photoinduced electron transfer.

MeSH terms

Electron Transport
Electrons*
Fluoroquinolones*
Positron-Emission Tomography

Substances

nadifloxacin
Fluoroquinolones