Molecular and functional characterization of viperin in golden pompano, Trachinotus ovatus

Lin Huang; Xiaowen Zhu; Jihui Kuang; Bohuan Li; Qing Yu; Mingzhu Liu; Bingzheng Li; Hui Guo; Pengfei Li

doi:10.1016/j.fsi.2023.109098

Molecular and functional characterization of viperin in golden pompano, Trachinotus ovatus

Fish Shellfish Immunol. 2023 Nov:142:109098. doi: 10.1016/j.fsi.2023.109098. Epub 2023 Sep 26.

Authors

Lin Huang¹, Xiaowen Zhu², Jihui Kuang³, Bohuan Li⁴, Qing Yu¹, Mingzhu Liu¹, Bingzheng Li⁵, Hui Guo⁶, Pengfei Li⁷

Affiliations

¹ Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Engineering Research Center for Fishery Major Diseases Control and Efficient Healthy Breeding Industrial Technology (GERCFT), Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences, Nanning, PR China.
² Guangdong Key Laboratory of Aquatic Animal Disease Prevention and Control and Healthy Aquaculture, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, College of Fishery, Guangdong Ocean University, Zhanjiang, PR China.
³ Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Engineering Research Center for Fishery Major Diseases Control and Efficient Healthy Breeding Industrial Technology (GERCFT), Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences, Nanning, PR China; School of Resources, Environment and Materials, Guangxi University, Nanning, PR China.
⁴ School of Resources, Environment and Materials, Guangxi University, Nanning, PR China.
⁵ Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Engineering Research Center for Fishery Major Diseases Control and Efficient Healthy Breeding Industrial Technology (GERCFT), Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences, Nanning, PR China; College of Food Science and Quality Engineering, Nanning University, Nanning, PR China.
⁶ Guangdong Key Laboratory of Aquatic Animal Disease Prevention and Control and Healthy Aquaculture, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, College of Fishery, Guangdong Ocean University, Zhanjiang, PR China. Electronic address: guoh@gdou.edu.cn.
⁷ Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Engineering Research Center for Fishery Major Diseases Control and Efficient Healthy Breeding Industrial Technology (GERCFT), Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences, Nanning, PR China; Guangdong Key Laboratory of Aquatic Animal Disease Prevention and Control and Healthy Aquaculture, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, College of Fishery, Guangdong Ocean University, Zhanjiang, PR China; School of Resources, Environment and Materials, Guangxi University, Nanning, PR China; College of Food Science and Quality Engineering, Nanning University, Nanning, PR China. Electronic address: pfli2016@gxas.cn.

PMID: 37758099
DOI: 10.1016/j.fsi.2023.109098

Abstract

The radical S-adenosyl methionine domain-containing protein 2 (RSAD2), also known as viperin, plays a momentous and multifaceted role in antiviral immunity. However, the function of viperin is uninvestigated in golden pompano, Trachinotus ovatus. In the present study, a viperin homolog, named To-viperin, was cloned and characterized from golden pompano, and its role in response to grouper iridovirus (SGIV) and nervous necrosis virus (NNV) infection was investigated. The whole open reading frame (ORF) of To-viperin was composed of 1050 bp and encoded a polypeptide of 349 amino acids with 70.66%-83.51% identity with the known viperin homologs from other fish species. A variable N-terminal domain, a highly conserved C-terminal domain, and a conserved middle radical SAM domain (aa 61-271) with the three-cysteine motif CxxCxxC was found in To-viperin sequence. Expression analysis showed that To-viperin was constitutively expressed in all tested organs and was located mainly in the ER of golden pompano cells. Treatments with SGIV, poly I: C, or NNV could induce the up-regulation of viperin to varying degrees. The ectopic expression of To-viperin in vitro significantly reduced the viral titer of SGIV and NNV. Furthermore, To-viperin overexpression enhanced the expression of IFNc, IRF3, and ISG15 genes as well as, to a lesser extent, the IL-6 gene. In summary, our results suggested that the function of viperin is likely to be conserved in fish specise, as observed in other vertebrates, shedding light on the evolutionary conservation of viperin.

Keywords: Immune response; Trachinotus ovatus; Viperin; Virus infection.

MeSH terms

Animals
Fish Diseases*
Fish Proteins / chemistry
Fishes
Immunity, Innate / genetics
Iridovirus*
Phylogeny

Substances

Fish Proteins