Structural insight into EV-A71 3A protein and its interaction with a peptide inhibitor

Yahui Liu; Panjing Lv; Wei Wang; Jiahai Zhang; Xi Zhou; Yang Qiu; Kun Cai; Haoran Zhang; Yuan Fang; Yan Li

doi:10.1016/j.virs.2023.09.004

Structural insight into EV-A71 3A protein and its interaction with a peptide inhibitor

Virol Sin. 2023 Dec;38(6):975-979. doi: 10.1016/j.virs.2023.09.004. Epub 2023 Sep 26.

Authors

Yahui Liu¹, Panjing Lv¹, Wei Wang², Jiahai Zhang³, Xi Zhou⁴, Yang Qiu⁴, Kun Cai⁵, Haoran Zhang⁶, Yuan Fang⁷, Yan Li⁸

Affiliations

¹ Department of Pathogen Biology, School of Basic Medicine, Tongji Medical College and State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Huazhong University of Science and Technology, Wuhan, 430030, China.
² Medical Subcenter of HUST Analytical & Testing Center, Huazhong University of Science and Technology, Wuhan, 430030, China.
³ MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230027, China.
⁴ State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.
⁵ Institute of Health Inspection and Testing, Hubei Provincial Center for Disease Control and Prevention (Hubei CDC), Wuhan, 430079, China. Electronic address: ckreal@163.com.
⁶ Department of Pathogen Biology, School of Basic Medicine, Tongji Medical College and State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Huazhong University of Science and Technology, Wuhan, 430030, China. Electronic address: hrzhang@outlook.com.
⁷ State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. Electronic address: fangyuan_125@163.com.
⁸ Department of Pathogen Biology, School of Basic Medicine, Tongji Medical College and State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Huazhong University of Science and Technology, Wuhan, 430030, China; Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. Electronic address: yanli@hust.edu.cn.

Abstract

•
Our results disclosed a dihelical structure of Enterovirus A71 3A_1–57 protein in apo form.
•
We depicted rigid helices and a unique flexible C-terminus for apo-form 3A_1–57.
•
This study revealed a competitive binding-based molecular mechanism underlying inhibition of dimeric 3A by ER-DRI.

Publication types

Letter

MeSH terms

Enterovirus A, Human*
Enterovirus Infections*
Humans
Peptides / pharmacology

Substances

Peptides