Long non-coding HOXA-AS3 contributes to osteosarcoma progression through the miR-1286/TEAD1 axis

J Orthop Surg Res. 2023 Sep 27;18(1):730. doi: 10.1186/s13018-023-04214-5.

Abstract

Long non-coding RNA (lncRNA) HOXA cluster antisense RNA 3 (HOXA-AS3) regulates the progression of several types of human malignancy. However, the role and potential mechanism of HOXA-AS3 in osteosarcoma (OS) remain unknown. In this study, upregulation of HOXA-AS3 was observed in OS tissues and cell lines and associated with poor clinical outcomes. Silencing of HOXA-AS3 significantly inhibited the proliferation, migration and invasion of OS cells in vitro and suppressed the tumorigenesis of OS cells in vivo. Furthermore, knockdown of HOXA-AS3 inhibited the proliferation and migration of human umbilical vein endothelial cells (HUVECs) and epithelial-to-mesenchymal transition (EMT) in OS. Further investigation of this mechanism revealed that HOXA-AS3 could directly upregulate the expression of TEAD1 via its competing endogenous RNA (ceRNA) activity on miR-1286. This study clarified the oncogenic roles of the HOXA-AS3/miR-1286/TEAD1 axis in OS progression, suggesting a novel therapeutic target for OS.

Keywords: HOXA cluster antisense RNA 3; MiR-1286; Osteosarcoma; TEA domain family mem.

MeSH terms

  • Bone Neoplasms* / genetics
  • Cell Line, Tumor
  • Cell Proliferation / genetics
  • Endothelial Cells / metabolism
  • Gene Expression Regulation, Neoplastic / genetics
  • Humans
  • MicroRNAs* / genetics
  • MicroRNAs* / metabolism
  • Osteosarcoma* / genetics
  • Osteosarcoma* / pathology
  • RNA, Long Noncoding* / genetics
  • RNA, Long Noncoding* / metabolism
  • TEA Domain Transcription Factors / genetics
  • TEA Domain Transcription Factors / metabolism

Substances

  • MicroRNAs
  • TEA Domain Transcription Factors
  • TEAD1 protein, human
  • RNA, Long Noncoding
  • MIRN1286 microRNA, human