Synthesis, physicochemical characteristics, cytocompatibility, and antibacterial properties of iron-doped biphasic calcium phosphate nanoparticles with incorporation of silver

Lei Nie; Pengbo Chang; Oseweuba Valentine Okoro; Musa Ayran; Oguzhan Gunduz; Kehui Hu; Tianwen Wang; Amin Shavandi

doi:10.1088/2057-1976/acfcbe

Synthesis, physicochemical characteristics, cytocompatibility, and antibacterial properties of iron-doped biphasic calcium phosphate nanoparticles with incorporation of silver

Biomed Phys Eng Express. 2023 Oct 11;9(6). doi: 10.1088/2057-1976/acfcbe.

Authors

Lei Nie^{1

2}, Pengbo Chang³, Oseweuba Valentine Okoro², Musa Ayran⁴, Oguzhan Gunduz⁴, Kehui Hu^{5

6}, Tianwen Wang⁷, Amin Shavandi²

Affiliations

¹ College of Life Sciences, Xinyang Normal University (XYNU), Xinyang 464000, People's Republic of China.
² Université libre de Bruxelles (ULB), École polytechnique de Bruxelles - BioMatter unit, Avenue F.D. Roosevelt, 50 - CP 165/61, 1050 Brussels, Belgium.
³ Department of Materials Engineering, Zhengzhou Technical College, Zhengzhou 450121, People's Republic of China.
⁴ Center for Nanotechnology and Biomaterials Application & Research (NBUAM), Marmara University, Istanbul, Turkey.
⁵ State Key Laboratory of Tribology, Tsinghua University, Beijing 100084, People's Republic of China.
⁶ Department of Mechanical Engineering, Tsinghua University, Beijing 100084, People's Republic of China.
⁷ College of Biology and Food Engineering, Anhui Polytechnic University, Wuhu 241000, Anhui, People's Republic of China.

PMID: 37748457
DOI: 10.1088/2057-1976/acfcbe

Abstract

The application of biphasic calcium phosphate (BCP) in tissue engineering and regenerative medicine has been widely explored due to its extensively documented multi-functionality. The present study attempts to synthesize a new type of BCP nanoparticles, characterised with favourable cytocompatibility and antibacterial properties via modifications in their structure, functionality and assemblage, using dopants. In this regard, this study initially synthesized iron-doped BCP (FB) nanoparticles with silver subsequently incorporated into FB nanoparticles to create a nanostructured composite (FB_Ag). The FB and FB_Agnanoparticles were then characterized using Fourier transform infrared spectroscopy, x-ray diffraction, ultraviolet-visible spectroscopy, and x-ray photoelectron spectroscopy. The results showed that silver was present in the FB_Agnanoparticles, with a positive correlation observed between increasing AgNO₃concentrations and increasing shape irregularity and reduced particle size distribution. Additionally, cell culture tests revealed that both FB and FB_Agnanoparticles were compatible with bone marrow-derived mesenchymal stem cells (hBMSCs). The antibacterial activity of the FB_Agnanoparticles was also tested using Gram-negativeE. coliand Gram-positiveS. aureus, and was found to be effective against both bacteria. The inhibition rates of FB_Agnanoparticles againstE. coliandS. aureuswere 33.78 ± 1.69-59.03 ± 2.95%, and 68.48 ± 4.11-89.09 ± 5.35%, respectively. These findings suggest that the FB_Agnanoparticles have potential use in future biomedical applications.

Keywords: antibacterial activity; biocompatibility; biphasic calcium phosphate; iron; silver nanoparticles.