Determination of bioactive flavonoids using β-cyclodextrin combined with chitosan-modified magnetic nanoparticles

Xiaoping Hai; Lei Ma; Yun Zhu; Zhi Yang; Xiaofen Li; Minghong Chen; Mingwei Yuan; Huabin Xiong; Yuntao Gao; Feng Shi; Lina Wang

doi:10.1016/j.carbpol.2023.121295

Determination of bioactive flavonoids using β-cyclodextrin combined with chitosan-modified magnetic nanoparticles

Carbohydr Polym. 2023 Dec 1:321:121295. doi: 10.1016/j.carbpol.2023.121295. Epub 2023 Aug 12.

Authors

Xiaoping Hai¹, Lei Ma², Yun Zhu², Zhi Yang², Xiaofen Li², Minghong Chen³, Mingwei Yuan⁴, Huabin Xiong⁵, Yuntao Gao⁶, Feng Shi², Lina Wang²

Affiliations

¹ School of Chemistry and Environment, Yunnan Minzu University, Kunming 650504, PR China; Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission, Ministry of Education, Yunnan Minzu University, Kunming 650504, PR China.
² School of Chemistry and Environment, Yunnan Minzu University, Kunming 650504, PR China.
³ Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission, Ministry of Education, Yunnan Minzu University, Kunming 650504, PR China.
⁴ National and Local Joint Engineering Research Center for Green Preparation Technology of Biobased Materials, Yunnan Minzu University, Kunming 650504, PR China.
⁵ School of Chemistry and Environment, Yunnan Minzu University, Kunming 650504, PR China; National and Local Joint Engineering Research Center for Green Preparation Technology of Biobased Materials, Yunnan Minzu University, Kunming 650504, PR China. Electronic address: xionghuabin@ynni.edu.cn.
⁶ School of Chemistry and Environment, Yunnan Minzu University, Kunming 650504, PR China; National and Local Joint Engineering Research Center for Green Preparation Technology of Biobased Materials, Yunnan Minzu University, Kunming 650504, PR China. Electronic address: 040343@ymu.edu.cn.

PMID: 37739528
DOI: 10.1016/j.carbpol.2023.121295

Abstract

To accurately determine flavonoids (rutin, quercetin or kaempferol), it is necessary to extract them from complex matrices. The ultrasound-assisted magnetic dispersion microsolid phase extraction technique has been predominantly used for separation and enrichment of the target analytes. The combination of magnetic chitosan nanoparticles and a deep eutectic supramolecular solvent (DESP) is likely to enhance the efficiency of flavonoid extraction from food. In this study, adsorbents were prepared by modifying chitosan with magnetic nanoparticles, and the eluent was a DESP derived from β-cyclodextrin and an organic acid. The successful preparation of these materials was confirmed by FTIR, XRD, FE-SEM and ¹H NMR. The extraction recovery rates exceeded 93 %, with limits of detection and quantitation ranging from 0.9 to 2.4 μg/L and 2.7 to 7.2 μg/L, respectively, and the flavonoid clearance rates for ABTS and DPPH radicals reached 100 %. Therefore, the integration of magnetic chitosan nanoparticles with the DESP provides a new and efficient method for the extraction of flavonoids while also presenting a potential application of the DESP in separations.

Keywords: Chitosan modification; Deep eutectic supramolecular solvents; Flavonoids; β-Cyclodextrin.

MeSH terms

Chitosan*
Flavonoids
Magnetite Nanoparticles*
Quercetin
beta-Cyclodextrins*

Substances

Flavonoids
Chitosan
Magnetite Nanoparticles
beta-Cyclodextrins
Quercetin