Copper indium selenium nanomaterials for photo-amplified immunotherapy through simultaneously enhancing cytotoxic T lymphocyte recruitment and M1 polarization of macrophages

Yining Chen; Yunan Yang; Xinai He; Xin Liu; Pengcheng Yu; Runru Liu; Liqi Wei; Biao Zhang; Tianshu Zou; Hongxiang Liu; Yuanqiang Li; Rui Chen; Yan Cheng

doi:10.1016/j.actbio.2023.09.033

Copper indium selenium nanomaterials for photo-amplified immunotherapy through simultaneously enhancing cytotoxic T lymphocyte recruitment and M1 polarization of macrophages

Acta Biomater. 2023 Nov:171:495-505. doi: 10.1016/j.actbio.2023.09.033. Epub 2023 Sep 20.

Authors

Yining Chen¹, Yunan Yang¹, Xinai He¹, Xin Liu¹, Pengcheng Yu², Runru Liu², Liqi Wei¹, Biao Zhang¹, Tianshu Zou¹, Hongxiang Liu¹, Yuanqiang Li¹, Rui Chen³, Yan Cheng⁴

Affiliations

¹ College of Life Science, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development Jilin Agricultural University, Changchun 130118, PR China.
² College of Science, Jilin Provincial Key Laboratory of Human Health Status Identification and Function Enhancement, Changchun University, Changchun 130022, PR China.
³ College of Science, Jilin Provincial Key Laboratory of Human Health Status Identification and Function Enhancement, Changchun University, Changchun 130022, PR China. Electronic address: chenr@ccu.edu.cn.
⁴ College of Life Science, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development Jilin Agricultural University, Changchun 130118, PR China. Electronic address: ycheng@jlau.edu.cn.

PMID: 37739250
DOI: 10.1016/j.actbio.2023.09.033

Abstract

Photoactivated immunotherapy has promising therapeutic efficacy for treating malignancies, especially metastatic tumors. In this study, an erythrocyte membrane-encapsulated copper indium selenium (RCIS) semiconductor nanomaterial was developed to eliminate primary and metastatic tumors, in which copper ions can induce chemodynamic performance, and the narrow band gap endows RCIS with the properties of near-infrared (NIR) light-activated photothermal and photodynamic amplified immunotherapy. Furthermore, RCIS can be used as a nanocarrier to form RNCIS nanoparticles (NPs) by loading NLG919, which blocks the indoleamine 2,3-dioxygenase-1. Under NIR light irradiation, RNCIS NPs release NLG919 at tumor sites via photothermal properties, thereby promoting the recruitment of cytotoxic T lymphocytes and M1 polarization of macrophages, targeting the activation and amplification of immune responses. Herein, in vitro and in vivo studies showed that RNCIS NPs effectively kill cancer cells and eliminate primary and metastatic tumors. Therefore, this study suggests that semiconductor nanomaterials with narrow bandgaps have great potential as photoimmunotherapy agents and NIR light-responsive nanocarriers for controlled release, providing a great paradigm for synergetic tumor photoimmunotherapy. STATEMENT OF SIGNIFICANCE: The Erythrocyte membrane-coated, NLG919-loaded copper indium selenium (RNCIS) semiconductor was designed for eliminating primary and metastatic tumors. RNCIS exhibits chemodynamic, photodynamic, and photothermal activated immunotherapy by inhibiting indoleamine 2,3-dioxygenase-1. This can enhance the recruitment of cytotoxic T lymphocyte and M1 polarization of macrophage, leading to higher synergetic photo-immune therapeutic efficacy.

Keywords: Cancer therapy; Indoleamine 2,3-dioxygenase-1; Photoelectronic nanomaterials; Photoimmunotherapy; Reactive oxygen species.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor
Copper / pharmacology
Humans
Immunotherapy
Indium
Indoleamine-Pyrrole 2,3,-Dioxygenase
Macrophages
Nanoparticles* / therapeutic use
Nanostructures*
Neoplasms* / pathology
Selenium* / pharmacology
T-Lymphocytes, Cytotoxic / pathology

Substances

Selenium
Copper
Indium
Indoleamine-Pyrrole 2,3,-Dioxygenase