Nanopore PCR-cDNA sequencing of the biocontrol isolate Aspergillus flavus AF36 (NRRL 18543) informs gene annotation

Microbiol Resour Announc. 2023 Oct 19;12(10):e0052723. doi: 10.1128/MRA.00527-23. Epub 2023 Sep 20.

Abstract

Toxic molds in the Aspergillus genus synthesize carcinogenic aflatoxins which contaminate crops. The widely applied biocontrol isolate Aspergillus flavus AF36 (NRRL 18543) has a high-quality public genome but lacks corresponding gene annotations. We generated high-quality gene predictions for this isolate by using long-read Nanopore PCR-cDNA sequencing.

Keywords: Aspergillus; Nanopore; aflatoxin; annotation; transcription.