IL1RL1 polymorphisms rs12479210 and rs1420101 are associated with increased lung cancer risk in the Chinese Han population

Qi Li; Chan Zhang; Yujing Cheng; Xin Yang; Wanlu Chen; Kunhua He; Mingwei Chen

doi:10.3389/fgene.2023.1183528

IL1RL1 polymorphisms rs12479210 and rs1420101 are associated with increased lung cancer risk in the Chinese Han population

Front Genet. 2023 Aug 31:14:1183528. doi: 10.3389/fgene.2023.1183528. eCollection 2023.

Authors

Qi Li^{1

2}, Chan Zhang², Yujing Cheng², Xin Yang², Wanlu Chen², Kunhua He³, Mingwei Chen¹

Affiliations

¹ Department of Respiratory Medicine, The First Affiliated Hospital of School of Medicine of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
² Department of Blood Transfusion, The First People's Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, Kunming, China.
³ Department of Blood Transfusion, Qujing No. 1 Hospital, Qujing, China.

Abstract

Background: Lung cancer is one of the most common human malignant diseases. In this study, we aimed to explore the association between IL1RL1 genetic polymorphisms and lung cancer risk in the Chinese Han population. Methods: We selected and genotyped six SNPs in the IL1RL1 gene using the Agena MassARRAY system in 507 lung cancer patients and 507 healthy controls. The association between IL1RL1 variants and lung cancer risk was assessed using logistic regression to calculate odds ratios (ORs) and 95% confidence intervals (CIs). Multi-factor dimensionality reduction (MDR) was used to analyze the impact of SNP-SNP interactions on the risk of lung cancer. Results: The results of overall analysis indicated that rs12479210 (T vs. C: OR = 1.42, FDR-p = 0.002; TC vs. CC: OR = 1.70, FDR-p < 0.0001; TT vs. CC: OR = 1.77, FDR-p = 0.032; TT-TC vs. CC: OR = 1.71, FDR-p = 0.001; additive: OR = 1.44, FDR-p = 0.001) and rs1420101 (T vs. C: OR = 1.31, FDR-p = 0.036; TT-TC vs. CC: OR = 1.42, FDR-p = 0.031; additive: OR = 1.30, FDR-p = 0.030) were associated with an increased the risk of lung cancer among the Chinese Han population. Stratified analysis also found the association between these two SNPs and lung cancer risk. However, there were no significant association observed between the other four SNPs (rs3771180, rs3771175, rs10208293, and rs10197862) in IL1RL1 and lung cancer risk. Furthermore, MDR analysis showed that rs12479210 was the best single model with the highest testing accuracy (0.566) and perfect CVC (10/10) for predicting lung cancer risk. The expression level of the IL1RL1 gene is lower in lung cancer tissue than normal tissue, and there are significant differences in the expression levels of IL1RL1 between rs12479210 and rs1420101 genetypes in lung cancer tissue (p < 0.05). Conclusion: Our findings suggest that IL1RL1 genetic variants (rs12479210 and rs1420101) are associated with an increased lung cancer risk in the Chinese Han population. These risk variants may serve as biomarkers for the prevention and treatment of lung cancer.

Keywords: IL1RL1; case-control study; genetic; lung cancer; polymorphism.

Grants and funding

This work was supported by the Applied Basic Research Foundation of Yunnan Province (CN) (grant number: 2017FE468 (−125) and 202001AY070001-111 and KUST-KH2022028Y), the Open Project of the Clinical Medicine Center of the First People’s Hospital of Yunnan Province (grant number: 2021LCZXXF-XY12 and 2022LCZXKF-XY04), CAMS Innovation Fund for Medical Sciences (CIFMS) (grant number: 2016-I2M), and Yunnan Province Clinical Center for Hematologic Disease and Yunnan Province Clinical Research Center for Hematologic Disease.