A novel mechanism of enhanced PCBs degradation associated with nitrogen in the rhizosphere of the wetland plant Myriophyllum aquaticum

Abstract

Co-contamination of polychlorinated biphenyls (PCBs) and nitrogen (N) is widespread. Here, N removal and PCBs degradation were investigated in constructed wetlands populated with Myriophyllum aquaticum, and the role of N in PCBs degradation was explored as well. Nearly 97% of N was removed in the planted system, whereas less than 40% was removed in the plant-free system. Compared to the treatment with plants and no N amendment, N addition enhanced plant growth by 31.9% and PCBs removal by 9.90%. PCBs attenuation was mainly attributed to microbial degradation rather than plant uptake. Using DNA stable-isotope probing, 26 operational taxonomic units were identified across all treatments, of which 25 were linked to PCBs degradation for the first time. Some PCB-degraders were associated with nitrification/denitrification and were significantly enriched in the treatment that included both plants and N application, indicating that PCBs degradation was promoted by recruiting ammonia-oxidising and denitrifying microbes with PCBs metabolic ability. This was confirmed by the higher A13/A12 ratios for the bphC, amoA, and nirK genes and their significant positive correlations. Overall, the findings clarify the novel mechanism by which N promotes PCBs degradation in constructed wetlands and offers a theoretical basis for efficiently removing inorganic elements and persistent organic pollutants.

Keywords: Ammonia; DNA-SIP; Myriophyllum aquaticum; PCB-degrading bacteria; Polychlorinated biphenyls.