Effect of cryopreservation and capacitation on expression patterns of ATP synthesis associated genes in bubaline spermatozoa

B V S Sai Kiran; C H Srinivasa Prasad; B R Naik; K Aswani Kumar; S Lavanya; Tej Nikhil Kumar; Iqbal Hyder

doi:10.1016/j.jtherbio.2023.103704

Effect of cryopreservation and capacitation on expression patterns of ATP synthesis associated genes in bubaline spermatozoa

J Therm Biol. 2023 Oct:117:103704. doi: 10.1016/j.jtherbio.2023.103704. Epub 2023 Sep 9.

Authors

B V S Sai Kiran¹, C H Srinivasa Prasad², B R Naik³, K Aswani Kumar⁴, S Lavanya¹, Tej Nikhil Kumar¹, Iqbal Hyder⁵

Affiliations

¹ Department of Veterinary Physiology, NTR College of Veterinary Science, Gannavaram, Sri Venkateswara Veterinary University, Andhra Pradesh, 521102, India.
² Department of Veterinary Physiology, NTR College of Veterinary Science, Gannavaram, Sri Venkateswara Veterinary University, Andhra Pradesh, 521102, India. Electronic address: spchigurupati@rediffmail.com.
³ Department of Veterinary Physiology, College of Veterinary Science, Tirupati, Sri Venkateswara Veterinary University, Andhra Pradesh, 517502, India.
⁴ Department of Veterinary Biochemistry, NTR College of Veterinary Science, Gannavaram, Sri Venkateswara Veterinary University, Andhra Pradesh, 521102, India.
⁵ Department of Veterinary Physiology and Biochemistry, College of Veterinary Science, Garividi, Sri Venkateswara Veterinary University, Andhra Pradesh, 535101, India. Electronic address: iqbvet@gmail.com.

PMID: 37714111
DOI: 10.1016/j.jtherbio.2023.103704

Abstract

This study investigated the expression patterns of certain ATP synthesis associated genes (GAPDHS, PGK2, ENO4 and MDH2) in fresh and frozen-thawed bubaline spermatozoa vis-à-vis capacitation. Eight adult Murrah buffalo bulls, aged 5-7 years, were randomly selected from a bull farm and fresh semen was collected once a week for four weeks (n = 32) from the selected bulls. Simultaneously, frozen straws of the same bulls were collected (n = 32, four straws were pooled per sample). The samples were divided into four groups i.e., fresh non capacitated (control/group 1), fresh capacitated (group 2), frozen non capacitated (group 3) and frozen capacitated (group 4). In all groups mRNA extraction was done. The mRNA expression of GAPDHS was significantly higher in group 3 compared to the other groups, whereas the expression of ENO4 was significantly up-regulated in group 2 and 3 compared to the group 4. Similarly, the mRNA expression of PGK2 was significantly up-regulated in group 3 whereas in case of MDH2, it was significantly higher in group 2, 3 and 4 compared group 1. It can be concluded from our study that freezing and capacitation has a significant effect on the expression dynamics of the ATP synthesis-associated genes warranting necessary interventions in handling procedures to minimise the adverse effect of freezing on ATP synthesis process to ensure persistence of fertilizing ability of sperm.

Keywords: ATP synthesis gene; Capacitation; Frozen-thawed; Murrah buffalo; Semen.