A flexible and wearable three-electrode electrochemical sensing system consisting of a two-in-one enzyme-mimic working electrode

Yanan Wang; Yali Liu; Xiao Wang; Xiyue Cao; Jianfei Xia; Zonghua Wang

doi:10.1016/j.aca.2023.341688

A flexible and wearable three-electrode electrochemical sensing system consisting of a two-in-one enzyme-mimic working electrode

Anal Chim Acta. 2023 Oct 16:1278:341688. doi: 10.1016/j.aca.2023.341688. Epub 2023 Aug 4.

Authors

Yanan Wang¹, Yali Liu², Xiao Wang¹, Xiyue Cao³, Jianfei Xia⁴, Zonghua Wang¹

Affiliations

¹ College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Instrumental Analysis Center of Qingdao University, Qingdao University, Qingdao, 266071, PR China.
² Qingdao Hospital, University of Health and Rehabilitation Sciences (Qingdao Municipal Hospital), Qingdao, 266071, PR China.
³ College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Instrumental Analysis Center of Qingdao University, Qingdao University, Qingdao, 266071, PR China. Electronic address: caoxiyue@qdu.edu.cn.
⁴ College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Instrumental Analysis Center of Qingdao University, Qingdao University, Qingdao, 266071, PR China. Electronic address: xiajianfei@126.com.

PMID: 37709441
DOI: 10.1016/j.aca.2023.341688

Abstract

In this work, a wearable and flexible three-electrode electrochemical sensing system (TESS) by using a two-in-one enzyme-mimic working electrode (TIOWE) is reported. The integrated three-electrode, including working electrodes, reference electrodes, and counter electrodes are formed by transfer printing of Ni₂P-based composite electrode ink (Ni₂P/G ink), Ag/AgCl ink, and carbon ink onto PDMS substrate, respectively. The Ni₂P/G ink-based working electrodes have both good conductivity and enzyme-mimic catalytic activity towards glucose. Under optimized conditions, the TIOWE-TESS has a low detection limit of 0.37 μM and wide linear ranges of 0.001 mM-0.1 mM and 0.1 mM-1.4 mM. Furthermore, the TIOWE-TESS has good applicability in serum samples and reveals remarkable electrochemical performance at fluctuant working temperatures. The proposed TIOWE-TESS can be integrated on a waterproof bandage to fabricate a skin-friendly patch device for sweet glucose monitoring, which highlights its potential applications in flexible and wearable commercial devices for health-monitoring.

Keywords: Enzyme-mimic electrode; Ni(2)P/G electrode ink; Sweat glucose; Wearable device; flexible electrochemical biosensing.

MeSH terms

Blood Glucose Self-Monitoring*
Blood Glucose*
Candy
Electrodes
Glucose

Substances

Blood Glucose
Glucose