Computer vision-assisted smartphone microscope imaging digital immunosensor based on click chemistry-mediated microsphere counting technology for the detection of aflatoxin B1 in peanuts

Meijie Ren; Yongzhen Dong; Jianlong Wang; Jianhan Lin; Lijie Qu; Yang Zhou; Yiping Chen

doi:10.1016/j.aca.2023.341687

Computer vision-assisted smartphone microscope imaging digital immunosensor based on click chemistry-mediated microsphere counting technology for the detection of aflatoxin B₁ in peanuts

Anal Chim Acta. 2023 Oct 16:1278:341687. doi: 10.1016/j.aca.2023.341687. Epub 2023 Aug 4.

Authors

Meijie Ren¹, Yongzhen Dong¹, Jianlong Wang², Jianhan Lin³, Lijie Qu⁴, Yang Zhou¹, Yiping Chen⁵

Affiliations

¹ College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
² College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, Shaanxi, China.
³ Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing, 100083, China.
⁴ Key Laboratory of Quality & Safety Analysis-Testing for Agro-Products and Food of Hebei Province, Hebei North University, No. 11 South Diamond Road, Zhangjiakou, 075000, China.
⁵ College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China. Electronic address: chenyiping@mail.hzau.edu.cn.

PMID: 37709427
DOI: 10.1016/j.aca.2023.341687

Abstract

Aflatoxin B₁ is a carcinogenic contaminant in food or feed, and it poses a serious health risk to humans. Herein, a computer vision-assisted smartphone microscope imaging digital (SMID) immunosensor based on the click chemistry-mediated microsphere counting technology was designed for the detection of aflatoxin B₁ in peanuts. In this SMID immunosensor, the modified polystyrene (PS) microspheres were used as the signal probes and were recorded by a smartphone microscopic imaging system after immunoreaction and click chemistry reaction. The number of PS probes is adjusted by aflatoxin B₁. The customized computer vision procedure was used to efficiently identify and count the obtained PS probes. This SMID immunosensor enables sensitive detection of aflatoxin B₁ with a linear range from 0.001 ng/mL to 500 ng/mL, providing a simple, sensitive, and portable tool for food safety supervision.

Keywords: Aflatoxin B(1); Click chemistry; Computer vision; Digital immunoassay; Microsphere counting technology; Smartphone microscope imaging.

MeSH terms

Aflatoxin B1
Arachis*
Biosensing Techniques*
Click Chemistry
Humans
Immunoassay
Microspheres
Smartphone
Technology

Substances

Aflatoxin B1