Effect of different irrigation methods on the toxicity and bioavailability of microcystin-LR to lettuce and carrot

Abstract

The use of cyanobacteria-polluted water for irrigation has become an increasing concern due to the potential contamination of microcystins (MCs). However, the effects of MCs on plant performance and food safety under different irrigation methods are not well understood. In this study, we investigated the effects of microcystin-LR (MC-LR) on the growth, food quality, and safety of lettuce and carrot using four irrigation methods (spray irrigation and three types of drip irrigation with different distances from the plant stem). Our results showed that exposure to 10 μg L^-1 MC-LR negatively affected plant growth and food quality in treatments with spray irrigation (TS) and drip irrigation directly to the stem (TD0), but not in treatments with drip irrigation away from the plant stem (TD10 and TD20). Using soil as a filtration system, the bioavailability of MC-LR in soil was reduced in TD10 and TD20, resulting in less bioaccumulation in plant edible tissues. The estimated daily intake (EDI) values of TS and TD0 in both lettuce and carrot cultivation exceeded the tolerable daily intake (TDI) limit proposed by WHO, whereas the EDI values of TD10 and TD20 could be effectively reduced below the TDI limit. This study highlights the importance of drip irrigation away from the plant stem as a practical measure to mitigate the effects of cyanobacteria-polluted water in agricultural production.

Keywords: Bioaccumulation; Cyanobacterial toxins; Drip irrigation; Food quality; Human health risk; Plant growth.