Enhanced tight junction in Caco-2 cells by the pretreatment with Lactobacillus johnsonii strain MG

Yuying Bai; Kazuhiko Miyanaga; Naoyuki Yamamoto

doi:10.1093/bbb/zbad131

Enhanced tight junction in Caco-2 cells by the pretreatment with Lactobacillus johnsonii strain MG

Biosci Biotechnol Biochem. 2023 Nov 21;87(12):1532-1536. doi: 10.1093/bbb/zbad131.

Authors

Yuying Bai¹, Kazuhiko Miyanaga^{1

2}, Naoyuki Yamamoto¹

Affiliations

¹ School of Life Science and Technology, Tokyo Institute of Technology, Yokohama, Kanagawa, Japan.
² Department of Infection and Immunity, School of Medicine, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-Shi, Tochigi, Japan.

PMID: 37704400
DOI: 10.1093/bbb/zbad131

Abstract

We investigated roles of Lactobacillus johnsonii MG (MG) isolated from mice with interaction with tight junction on gut barrier function with Caco-2 cell model. Pretreatment with MG enhanced barrier function and showed protective effect against Enterococcus faecium provided damage. MG treatment increased the gene expressions of transcriptional regulator NFKB and major tight junction protein, ZO-1.

Keywords: Lactobacillus johnsonii MG; Caco-2 cells; gut barrier function; tight junction.

MeSH terms

Animals
Caco-2 Cells
Humans
Intestinal Mucosa / metabolism
Lactobacillus johnsonii*
Mice
Tight Junction Proteins
Tight Junctions*

Substances

Tight Junction Proteins