Fluorescence microscope light source based on integrated LED

Jianchen Zi; Hai Bi

doi:10.1038/s41377-023-01245-9

Fluorescence microscope light source based on integrated LED

Light Sci Appl. 2023 Sep 12;12(1):224. doi: 10.1038/s41377-023-01245-9.

Authors

Jianchen Zi¹, Hai Bi²

Affiliations

¹ Jihua Laboratory, No.28 Huandao South Road, Nanhai district, 528200, Foshan, China.
² Jihua Laboratory, No.28 Huandao South Road, Nanhai district, 528200, Foshan, China. bihai@jihualab.ac.cn.

Abstract

An LED-integrated excitation cube (LEC) was designed to address the limitations of conventional fluorescence lamps. The LEC has a decentralized structure, high optical power density, and efficient illumination. The optical efficiency of LECs is 1-2 orders of magnitude higher than that of mercury lamps, enabling high-quality fluorescence imaging with spectral coverage from UV to red. LECs can be easily installed on commercial fluorescence microscopes by replacing conventional fluorescence filter cubes, and a built-in LEC driver can identify the types of LEDs in different spectral bands to adopt optimal operating conditions.