PEDV promotes the differentiation of CD4+T cells towards Th1, Tfh, and Treg cells via CD103+DCs

Haoyuan Yu; Guohui Chen; Tao Zhang; Xin Huang; YaBin Lu; Maolin Li; Shuxian Li; Caiying Wang; Baoyu Li; Yunhang Zhang; Guangliang Liu; Yuguang Fu

doi:10.1016/j.virol.2023.109880

PEDV promotes the differentiation of CD4⁺T cells towards Th1, Tfh, and Treg cells via CD103⁺DCs

Virology. 2023 Oct:587:109880. doi: 10.1016/j.virol.2023.109880. Epub 2023 Sep 7.

Authors

Haoyuan Yu¹, Guohui Chen¹, Tao Zhang¹, Xin Huang¹, YaBin Lu¹, Maolin Li¹, Shuxian Li¹, Caiying Wang¹, Baoyu Li¹, Yunhang Zhang¹, Guangliang Liu¹, Yuguang Fu²

Affiliations

¹ State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
² State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China. Electronic address: fuyuguang@caas.cn.

PMID: 37696054
DOI: 10.1016/j.virol.2023.109880

Abstract

Porcine epidemic diarrhea virus (PEDV) can infect all ages of pigs, particularly newborn piglets with a mortality almost reaching to 80-100%, causing significant economic losses to the global pig industry. The mucosal immune response is crucial for PEDV prevention, in which specific dendritic cells (DCs) and differentiated T cells play vital roles. In this study, CD103⁺DCs were differentiated successfully with retinoic acid (RA) treatment in vitro. PEDV could not replicate efficiently in differentiated CD103⁺DCs but could promote maturation of CD103⁺DCs by up-regulating the expression of SLA-DR, CD1a, CD86, and cytokines of IL-1β and IL-10. In addition, PEDV-infected CD103⁺DCs and CD4⁺T cells were co-cultured, and the results showed that the differentiation of CD4⁺T cells toward Th1, Tfh, and Treg, but not Th2. These results demonstrate that PEDV-infected CD103⁺DCs could promote the differentiation of CD4⁺T cells, which provided the basis for further study of mucosal response induced by PEDV via CD103⁺DCs.

Keywords: CD103(+)DCs; CD4(+)T differentiation; PEDV.