To Evaluate the Antimicrobial Activity of PRP and PRF with and without Nanosilver

Adil Zafar; Vartika Tripathi; Mariyam Khan; Keshav K Manglam; Preeti Rastogi; Abdullah M Almotreb

doi:10.4103/jpbs.jpbs_72_23

To Evaluate the Antimicrobial Activity of PRP and PRF with and without Nanosilver

J Pharm Bioallied Sci. 2023 Jul;15(Suppl 2):S892-S894. doi: 10.4103/jpbs.jpbs_72_23. Epub 2023 Jul 11.

Authors

Adil Zafar¹, Vartika Tripathi², Mariyam Khan³, Keshav K Manglam⁴, Preeti Rastogi⁵, Abdullah M Almotreb⁶

Affiliations

¹ Private Practitioner, Conservative Dentistry and Endodontics, Balia, Uttar Pradesh, India.
² Department of Orthodontics, Ahmedabad Dental College and Hospital, Ahmedabad, Gujarat, India.
³ Department of Conservative Dentistry and Endodontics, Career Postgraduate Institute of Dental Sciences and Hospital, Lucknow, Uttar Pradesh, India.
⁴ Department of Conservative Dentistry and Endodontics, Maharana Pratap Dental College, Kanpur, Uttar Pradesh, India.
⁵ Department of Conservative Dentistry and Endodontics, Sardar Patel Postgraduate Institute of Dental and Medical Sciences, Lucknow, Uttar Pradesh, India.
⁶ General Practitioner, MoH Riyadh Specialised Dental Center, Riyadh, KSA.

Abstract

Aim: To evaluate the antimicrobial activity of PRP and PRF with and without nanosilver.

Materials and methods: The materials were tested in powdered form is nanosilver. The nanosilver particles was mixed to form with PRP and PRF so as to placed in a wells followed the groups are experimental groups; Group I: PRP + nanosilver particles, Group II: PRF + nanosilver and control group: PRP and PRF and normal saline. Silver nanoparticles was tested at concentrations of 50 μ gram per mL. The powder was prepared for each group with identical amount of the powder (milligram/mg) and then mixed with 1 milliliter liquid. The plates are then incubated at 37°C under appropriate atmospheric conditions (80% N₂, 10% CO_2, 10% H₂) for 24 hours, 48 hours, and 72 hours under anaerobic conditions in a CO₂ incubator. The diameters of the zones of bacterial and fungal growth inhibition around the wells containing the test substances are then recorded after the period of incubation. The inhibitory zone determined in millimeter by measuring scale the shortest distance between the outer margin of the well and initial microbial as well as fungal growth. The experiments were performed 20 times and the mean and standard deviations of the inhibitory zones were calculated.

Result: Platelet rich fibrin is mixed with nanosilver particles showed higher antimicrobial efficacy than platelet rich plasma with nanosilver and simple platelet rich plasma and platelet rich fibrin are equivalent when it is placed against the anaerobic bacteria E.faecalis and yeast like fungi Candida albicans, respectively.

Conclusion: Groups presented with antimicrobial efficacy in this order- Group IV > Group II > Group III > Group I.

Keywords: Nanosilver; platelet rich fibrin; platelet rich plasma.