Spectroscopic and molecular modeling studies of binding interaction between the new complex of yttrium and 1,10-phenanthroline derivatives with DNA and BSA

Mahsa Khorshidi; Saeid Asadpour; Zahra Aramesh-Boroujeni; Masoumeh Kooravand; Maryam Mobini Dehkordi

doi:10.3389/fchem.2023.1231504

Spectroscopic and molecular modeling studies of binding interaction between the new complex of yttrium and 1,10-phenanthroline derivatives with DNA and BSA

Front Chem. 2023 Aug 24:11:1231504. doi: 10.3389/fchem.2023.1231504. eCollection 2023.

Authors

Mahsa Khorshidi¹, Saeid Asadpour¹, Zahra Aramesh-Boroujeni², Masoumeh Kooravand¹, Maryam Mobini Dehkordi³

Affiliations

¹ Department of Chemistry, Faculty of Sciences, Shahrekord University, Shahrekord, Iran.
² Department of Chemistry, University of Isfahan, Isfahan, Iran.
³ Cardiology Department, Shahrekord University of Medical Sciences, Shahrekord, Iran.

Abstract

In this study, the 4,9 diazafluoren-9-one ligand and [Y(Daf)₂Cl₃.OH₂] complex were synthesized. The interaction of this complex with DNA and bovine serum albumin (BSA) was investigated by UV-vis and fluorescence spectroscopy. The molecular docking method was used to confirm the experimental results, investigate the type of interaction, and determine the binding site. The binding constant and Stern-Volmer constant were calculated using spectroscopy techniques. The binding constant of the Y-complex with DNA and BSA obtained using the UV-vis technique was 1.61 × 10⁵ M^-1 and 0.49 × 10⁵ M^-1, while that obtained using the fluorescence method was 3.39 × 10⁵ M^-1 and 3.63 × 10⁵ M^-1, respectively. The results of experimental and theoretical data showed that the interaction between the yttrium complex and DNA and BSA is driven by the hydrogen bond and van der Waals interaction, respectively. The yttrium complex communicates with DNA via the groove interaction. This complex has high binding energy with bovine serum albumin. In addition, the molecular docking results showed that the complex binds to the IIA subdomain of BSA (site I). Finally, anticancer activity of the yttrium complex was studied on MCF-7 and A549 cell lines by using the MTT method. The IC₅₀ values obtained showed that the yttrium complex possesses anticancer activity.

Keywords: BSA interaction; DNA interaction; anticancer; molecular docking; yttrium complex.

Publication types

Retracted Publication