CEACAM expression in an in-vitro prostatitis model

Irina Kube-Golovin; Mykola Lyndin; Marc Wiesehöfer; Gunther Wennemuth

doi:10.3389/fimmu.2023.1236343

CEACAM expression in an in-vitro prostatitis model

Front Immunol. 2023 Aug 25:14:1236343. doi: 10.3389/fimmu.2023.1236343. eCollection 2023.

Authors

Irina Kube-Golovin¹, Mykola Lyndin^{1

2}, Marc Wiesehöfer¹, Gunther Wennemuth¹

Affiliations

¹ University Hospital Essen, Department of Anatomy, Essen, Germany.
² Academic and Research Medical Institute, Department of Pathology, Sumy State University, Sumy, Ukraine.

Abstract

Background: Prostatitis is an inflammatory disease of the prostate gland, which affects 2-16% of men worldwide and thought to be a cause for prostate cancer (PCa) development. Carcinoembryogenic antigen-related cell adhesion molecules (CEACAMs) are deregulated in inflammation and in PCa. The role of CEACAMs in prostate inflammation and their possible contribution to the malignant transformation of prostate epithelial cells is still elusive. In this study, we investigated the expression of CEACAMs in an in-vitro prostatitis model and their potential role in malignant transformation of prostate epithelial cells.

Methods: Normal prostate epithelial RWPE-1 cells were treated with pro-inflammatory cytokines to achieve an inflammatory state of the cells. The expression of CEACAMs and their related isoforms were analyzed. Additionally, the expression levels of selected CEACAMs were correlated with the expression of malignancy markers and the migratory properties of the cells.

Results: This study demonstrates that the pro-inflammatory cytokines, tumor necrosis factor alpha (TNFα) and interferon-gamma (IFNγ), induce synergistically an up-regulation of CEACAM1 expression in RWPE-1 cells, specifically favoring the CEACAM1-L isoform. Furthermore, overexpressed CEACAM1-L is associated with the deregulated expression of JAK/STAT, NFκB, and epithelial-mesenchymal transition (EMT) genes, as well as an increased cell migration.

Conclusion: We postulate that CEACAM1 isoform CEACAM1-4L may synergistically contribute to inflammation-induced oncogenesis in the prostate.

Keywords: CEACAM; CEACAM1; inflammation; prostate cancer; prostatitis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Transformation, Neoplastic
Cytokines
Humans
Inflammation
Male
Prostate
Prostatitis*
Transcription Factors
Tumor Necrosis Factor-alpha

Substances

Tumor Necrosis Factor-alpha
Transcription Factors
Cytokines

Grants and funding

The project was funded by the DFG to GW (Si 1558/6-1). ML was funded by Philipp Schwartz-Initiative Research Grant CEACAM1 to CEACAM21 expression pattern in normal and pathological tissue”. MW was funded by DFG WI 5802/2-1.