Emergence of yellowing disease in cucurbitaceous vegetables caused by Crinivirus and Polerovirus in India

Krishnan Nagendran; Shweta Kumari; Sudhakar Pandey; Pradip Karmakar; Tribhuvan Chaubey; Rajesh Kumar; S Vignesh; Gandhi Karthikeyan; Tusar Kanti Behera

doi:10.1016/j.virol.2023.109876

Emergence of yellowing disease in cucurbitaceous vegetables caused by Crinivirus and Polerovirus in India

Virology. 2023 Oct:587:109876. doi: 10.1016/j.virol.2023.109876. Epub 2023 Sep 2.

Authors

Krishnan Nagendran¹, Shweta Kumari², Sudhakar Pandey³, Pradip Karmakar³, Tribhuvan Chaubey³, Rajesh Kumar³, S Vignesh⁴, Gandhi Karthikeyan⁴, Tusar Kanti Behera⁵

Affiliations

¹ Division of Crop Protection, ICAR-Indian Institute of Vegetable Research, Varanasi, 221305, India. Electronic address: krishnagendra@gmail.com.
² Division of Crop Protection, ICAR-Indian Institute of Vegetable Research, Varanasi, 221305, India.
³ Division of Crop Improvement, ICAR-Indian Institute of Vegetable Research, Varanasi, 221305, India.
⁴ Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, 641003, India.
⁵ ICAR-Indian Institute of Vegetable Research, Varanasi, 221305, India.

PMID: 37688923
DOI: 10.1016/j.virol.2023.109876

Abstract

Totally 102 symptomatic samples of cucurbitaceous vegetables showing yellowing were collected from fields of Uttar Pradesh and screened by RT-PCR assay for the presence of Crinivirus and Polerovirus. Among them, Crinivirus (16%) and Polerovirus (23%) were tested positive with the universal primer pairs. Based on the sequence analysis of amplified product, two Crinivirus (Cucurbit chlorotic yellows virus - CCYV and Cucurbit yellow stunting disorder virus - CYSDV) and two Polerovirus (Cucurbit aphid-borne yellows virus - CABYV and Luffa aphid-borne yellows virus - LABYV) species were characterized. Phylogenetic analysis revealed less genetic distance among the Indian isolates of CCYV, CYSDV and LABYV whereas CABYV closely related to Chinese isolates. To the best of our knowledge, this study documents infection of CCYV on cucumber, round melon and muskmelon; CYSDV on satputia and sponge gourd; CABYV on ivy gourd; and LABYV on ridge gourd, satputia and muskmelon for the first time in India.

Keywords: Aphid-borne virus; Cucurbits virus; Vegetable viruses; Whitefly-borne virus; Yellowing disease.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Aphids*
Crinivirus* / genetics
Luteoviridae* / genetics
Phylogeny
Vegetables