Melatonin receptor, GhCAND2-D5 motivated responding to NaCl signaling in cotton

Yuexin Zhang; Maohua Dai; Zhe Wu; Shuai Wang; Yapeng Fan; Kesong Ni; Xuke Lu; Xiaoyu Liu; Mengyue Liu; Wenhua Chen; Xiugui Chen; Delong Wang; Junjuan Wang; Lixue Guo; Lanjie Zhao; Xiuping Wang; Wuwei Ye

doi:10.1016/j.plaphy.2023.108001

Melatonin receptor, GhCAND2-D5 motivated responding to NaCl signaling in cotton

Plant Physiol Biochem. 2023 Oct:203:108001. doi: 10.1016/j.plaphy.2023.108001. Epub 2023 Sep 7.

Authors

Yuexin Zhang¹, Maohua Dai², Zhe Wu³, Shuai Wang¹, Yapeng Fan¹, Kesong Ni¹, Xuke Lu¹, Xiaoyu Liu¹, Mengyue Liu¹, Wenhua Chen¹, Xiugui Chen¹, Delong Wang¹, Junjuan Wang¹, Lixue Guo¹, Lanjie Zhao¹, Xiuping Wang⁴, Wuwei Ye⁵

Affiliations

¹ Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Anyang, Henan, 455000, China.
² Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Anyang, Henan, 455000, China; Dryland Farming Institute, Hebei Academy of Agricultural and Forestry Sciences, Hebei Key Laboratory of Crops Drought Resistance, Hengshui, 053000, Hebei, China.
³ Institute of Coastal Agriculture, Hebei Academy of Agriculture and Forestry Sciences, Tangshan, 063299, Hebei, China.
⁴ Institute of Coastal Agriculture, Hebei Academy of Agriculture and Forestry Sciences, Tangshan, 063299, Hebei, China. Electronic address: bhswxp@163.com.
⁵ Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Anyang, Henan, 455000, China. Electronic address: yew158@163.com.

PMID: 37688899
DOI: 10.1016/j.plaphy.2023.108001

Abstract

As a receptor for plant melatonin, CAND2/PMTR plays an important role in melatonin signaling. Most of the CANDs are membrane proteins and play indispensable roles in signal transduction. In this study, the CANDs from four cotton species were characterized, and the phylogenetic relationships, expression patterns, stress responses of cotton CANDs were analyzed by bioinformatics. Through the analysis of phylogenetic and protein structure, it was found that the CANDs in clade Ⅱ might function as cotton melatonin receptors, and most of the GhCANDs in clade Ⅱ were induced by melatonin. A putative cotton melatonin receptor, GhCAND2-D5, was functionally probed by gene silencing. The plants with silenced expression of this gene exhibited decreased salt tolerance. Protein interaction prediction identified that GhCAND2-D5 interacted with several membrane proteins and played an important role in melatonin signaling. This study provided a theoretical reference for further investigation of melatonin signaling in cotton.

Keywords: Abiotic stress; CANDIDATE G-PROTEIN COUPLED RECEPTOR; Cotton; Melatonin; Plant melatonin receptors.