Visualizing and Manipulating Olfactory Cilia Through Viral Delivery Coupled with En Face Imaging of Intact OE

Julien C Habif; Chao Xie; Jeffrey R Martens

doi:10.1007/978-1-0716-3425-7_1

Visualizing and Manipulating Olfactory Cilia Through Viral Delivery Coupled with En Face Imaging of Intact OE

Methods Mol Biol. 2023:2710:1-18. doi: 10.1007/978-1-0716-3425-7_1.

Authors

Julien C Habif^{1

2}, Chao Xie^{1

2}, Jeffrey R Martens^{3

4}

Affiliations

¹ Department of Pharmacology and Therapeutics, University of Florida College of Medicine, Gainesville, FL, USA.
² Center for Smell and Taste, University of Florida College of Medicine, Gainesville, FL, USA.
³ Department of Pharmacology and Therapeutics, University of Florida College of Medicine, Gainesville, FL, USA. martensj@ufl.edu.
⁴ Center for Smell and Taste, University of Florida College of Medicine, Gainesville, FL, USA. martensj@ufl.edu.

PMID: 37688720
DOI: 10.1007/978-1-0716-3425-7_1

Abstract

Olfactory cilia are the obligate transducers of the odorant signal, and thus their study has been a focus of investigation in the olfactory field. Various methodologies have been established to visualize the cilia of olfactory sensory neurons; however, these approaches are limited to static imaging and often lack the ability to resolve individual cilia projecting from solitary neurons in the postnatal mouse. Here we detail a procedure of the visualization of olfactory cilia by ectopic expression of fluorescently tagged proteins. The procedure can be used for the observation and manipulation of the olfactory cilia and ciliary proteins in both static and dynamic conditions.

Keywords: Cilia; En face imaging; FRAP; Intranasal delivery; Olfactory epithelium; TIRF.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
Cilia*
Diffusion Magnetic Resonance Imaging
Mice
Odorants
Olfactory Receptor Neurons*
Smell

Abstract

Publication types

MeSH terms

Grants and funding