Antihyperglycemic potential of the Lavandula stoechas aqueous extract via inhibition of digestive enzymes and reduction of intestinal glucose absorption

Amal Elrherabi; Mohamed Bouhrim; Rhizlan Abdnim; Ali Berraaouan; Abderrahim Ziyyat; Hassane Mekhfi; Abdelkhaleq Legssyer; Mohamed Bnouham

doi:10.1016/j.jaim.2023.100795

Antihyperglycemic potential of the Lavandula stoechas aqueous extract via inhibition of digestive enzymes and reduction of intestinal glucose absorption

J Ayurveda Integr Med. 2023 Sep-Oct;14(5):100795. doi: 10.1016/j.jaim.2023.100795. Epub 2023 Sep 6.

Authors

Amal Elrherabi¹, Mohamed Bouhrim¹, Rhizlan Abdnim¹, Ali Berraaouan¹, Abderrahim Ziyyat¹, Hassane Mekhfi¹, Abdelkhaleq Legssyer¹, Mohamed Bnouham²

Affiliations

¹ Laboratory of Bioresources, Biotechnology, Ethnopharmacology, and Health, Faculty of Sciences, Mohammed First University, Oujda B.P. 717, Morocco.
² Laboratory of Bioresources, Biotechnology, Ethnopharmacology, and Health, Faculty of Sciences, Mohammed First University, Oujda B.P. 717, Morocco. Electronic address: mbnouham@yahoo.fr.

Abstract

Background: Diabetes mellitus is a widespread metabolic disorder affecting global populations. Lavandula stoechas from Moroccan traditional medicine is used for its potential anti-diabetic effects.

Objective: This study aims to evaluate the antihyperglycemic impact of the aqueous extract of L. stoechas (AqLs) and explore its mechanisms.

Methods: The study employed a glucose tolerance test (OGTT) on normal and diabetic Wistar rats, administering AqLs at 150 mg/kg. In vitro, AqLs was tested against α-glucosidase and α-amylase activities, confirmed in vivo using normal and Allx-diabetic rats. The extract's impact on intestinal d-glucose absorption was assessed using the jejunum segment perfusion technique at 250 mg/kg in situ. Albino mice were used to assess toxicity.

Results: AqLs significantly reduced postprandial hyperglycemia (P < 0.001) due to glucose overload. It inhibited pancreatic α-amylase (IC50: 0.485 mg/mL) and intestinal α-glucosidase (IC50: 168 µg/mL) in vitro. Oral AqLs at 150 mg/kg reduced hyperglycemia induced by sucrose and starch in normal and diabetic rats. It also lowered (P < 0.001) intestinal glucose absorption in situ at 250 mg/kg. Oral acute toxicity tests on Albino mice indicated no adverse effects at different doses.

Conclusion: to summarize, L. stoechas has evident antihyperglycemic effects attributed to inhibiting intestinal glucose absorption and key monosaccharide digestion enzymes like α-amylase and α-glucosidase.

Keywords: Antihyperglycemic; In vitro; In vivo; Lavandula stoechas; α-amylase; α-glucosidase.